摘要
目的分析平滑肌22 alpha蛋白(SM22α)、转化生长因子-β_(1)(TGF-β_(1))在结直肠癌组织中的表达水平,并探讨其与肿瘤侵袭转移的关系。方法回顾性选取咸阳市中心医院2018年4月至2019年5月期间收治的104例结直肠癌患者为研究组,按照肿瘤侵袭转移情况分为侵袭转移组(n=31)和非侵袭转移组(n=73),并选取同期于我院接受治疗的87例良性结直肠肿瘤患者作为对照组。采用免疫组化S-P法检测并比较各组患者结直肠病变组织中SM22α、TGF-β_(1)表达水平,采用Spearman秩相关分析SM22α、TGF-β_(1)表达水平与肿瘤侵袭转移的关系,并采用受试者特征曲线(ROC)评估SM22α和TGF-β_(1)表达水平对结直肠癌患者肿瘤侵袭转移的评估效能。结果研究组患者的SM22α阳性表达率为48.08%,明显低于对照组的64.37%,TGF-β_(1)阳性表达率为69.23%,明显高于对照组的36.78%,差异均有统计学意义(P<0.05);C期/D期患者SM22α阳性表达率为32.61%,明显低于A期/B期的60.34%,TGF-β_(1)阳性表达率为80.43%,明显高于A期/B期的60.34%,差异均有统计学意义(P<0.05);侵袭转移组患者的SM22α阳性表达率为32.26%,明显低于非侵袭转移组的54.79%,TGF-β_(1)阳性表达率为87.10%,明显高于非侵袭转移组的64.38%,差异均有统计学意义(P<0.05);经Spearman秩相关分析结果显示,SM22α与肿瘤侵袭转移呈负相关(r=-0.471,P<0.05),TGF-β_(1)与肿瘤侵袭转移呈正相关(r=0.543,P<0.05);ROC分析结果显示,SM22α、TGF-β_(1)及联合检测肿瘤侵袭转移的ROC曲线下面积分别为0.663、0.690、0.731,检测灵敏度分别为80.3%、78.6%、84.5%,特异性分别为60.8%、60.0%、61.8%,并且联合检测的ROC曲线下面积高于单项检测,检测灵敏度和特异性高于单项检测。结论结直肠癌组织中SM22α表达减少,TGF-β_(1)表达增加,且与肿瘤侵袭转移密切相关,可用于初步诊断肿瘤侵袭转移,且两者联合检测诊断效能更好。
Objective To analyze the expression levels of smooth muscle 22 alpha protein(SM22α)and trans-forming growth factor-β_(1)(TGF-β_(1))in colorectal cancer tissues,and to explore their relationship with tumor invasion and metastasis.Methods A total of 104 patients with colorectal cancer admitted to Xianyang Central Hospital from April 2018 to May 2019 were retrospectively selected as the study group.According to the tumor invasion and metastasis,they were divided into invasive metastatic group(n=31)and non-invasive metastatic group(n=73).Eighty-seven patients with benign colorectal tumor who received treatment in the hospital during the same period were selected as the control group.The expression levels of SM22αand TGF-β_(1) in colorectal lesions were detected by immunohistochemical S-P method and compared between groups.Spearman rank correlation analysis was used to analyze the relationship between the expression levels of SM22αand TGF-β_(1) and tumor invasion and metastasis.Receiver characteristic curve(ROC)was used to evaluate the efficacy of SM22αand TGF-β_(1) expression in evaluating tumor invasion and metastasis in colorectal cancer patients.Results The positive expression rate of SM22αin the study group was 48.08%,which was significantly lower than 64.37%in the control group,and the positive expression rate of TGF-β_(1) was 69.23%,which was significantly higher than 36.78%in the control group,with statistically significant differences(P<0.05).The positive expression rate of SM22αin stage C/D was 32.61%,significantly lower than 60.34%in stage A/B,and the positive expression rate of TGF-β_(1) was 80.43%,significantly higher than 60.34%in stage A/B,with statistically significant difference(P<0.05).The positive expression rate of SM22αin invasive metastasis group was 32.26%,significantly lower than 54.79%in non-invasive metastasis group,and the positive expression rate of TGF-β_(1) was 87.10%,significantly higher than 64.38%in non-invasive metastasis group,with statistically significant differences
作者
张粉娟
董兵卫
沈江立
张渭波
罗小红
ZHANG Fen-juan;DONG Bing-wei;SHEN Jiang-li;ZHANG Wei-bo;LUO Xiao-hong(Department of Pathology,Xianyang Central Hospital,Xianyang 712000,Shaanxi,CHINA;Department of Pathology 1,Department of Anorectal Surgery,Xianyang Central Hospital,Xianyang 712000,Shaanxi,CHINA)
出处
《海南医学》
CAS
2022年第12期1497-1500,共4页
Hainan Medical Journal
基金
陕西省中医药管理局科研课题(编号:2019-GJ-JC111)。
