摘要
目的:探究黑麦草与樊庆生红球菌联合对不同浓度Pb污染土壤的修复效果,为土壤重金属修复研究提供参考。方法:使用一年生黑麦草进行室内培养试验,共设置6个处理,分别为土壤中无外源铅不接种菌液(CK)、土壤中无外源铅接种菌液(CK+R)、模拟农业污染土壤不接种菌液(C_(1))、模拟农业污染土壤接种菌液(C_(1)+R)、模拟林业污染土壤不接种菌液(C_(2))和模拟林业污染土壤接种菌液(C_(2)+R),每个处理均重复9次。培养时间共28 d,培养完成后取样进行铅含量测定。结果:在相同培养条件下,相较于CK、CK+R处理组中黑麦草地上部长势未有明显变化。比较C_(2)+R处理组与C_(2)组,可发现在模拟林业污染土壤中黑麦草根际接种菌液后,地上部生长受到一定程度的抑制。相较于CK、C_(1)、C_(2)对照组,CK+R、C_(1)+R、C_(2)+R实验组黑麦草地下部生长明显受到促进,增长率分别为18.5%、15.3%、22.4%。C_(1)+R、C_(2)+R实验组地上部Pb含量与C_(1)、C_(2)相比,分别增加了104%、30.25%,地下部Pb含量分别增长2.2%、2.3%。结论:在黑麦草根系接种樊庆生红球菌可有效促进黑麦草对于重金属铅的吸收,增强其植株抗逆性。樊庆生红球菌黑麦草联合修复技术可用于修复铅污染土壤,为修复土壤重金属污染提供新方法,为微生物菌剂的研发提供新思路。
Objective:To explore the effect of ryegrass combined with Rhodococcus qingshengii on remediation of Pb contaminated soil with different concentrations,and to provide basis for remediation of heavy metals in soil.Methods:The indoor culture experiment was carried out with one-year-old ryegrass.Six treatments were set up,including no exogenous lead in soil(CK),no exogenous lead in soil(CK+R),no inoculation in simulated agricultural contaminated soil(C_(1)),inoculation in simulated agricultural contaminated soil(C_(1)+R),no inoculation in simulated forestry contaminated soil(C_(2))and inoculation in simulated forestry contaminated soil(C_(2)+R).Each treatment was repeated nine times.The incubation time was 28 days,and the lead content was determined after the incubation.Results:Under the same culture conditions,compared with CK,the aboveground growth of ryegrass in(CK+R)treatment group did not change significantly.Comparing(C_(2)+R)treatment group with C_(2),it can be found that the aboveground growth of ryegrass was inhibited to a certain extent after inoculation in the rhizosphere of ryegrass in simulated forest contaminated soil.Compared with CK,C_(1) and C_(2) control groups,the growth of ryegrass underground in(CK+R),(C_(1)+R)and(C_(2)+R)groups was significantly promoted,with the growth rates of 18.5%,15.3%and 22.4%,respectively.Compared with C_(1) and C_(2),the content of Pb in aboveground part of(C_(1)+R)and(C_(2)+R)groups increased by 104%and 30.25%respectively,and the content of Pb in underground part increased by 2.2%and 2.3%,respectively.Conclusion:Inoculation of Rhodococcus qingshengii in ryegrass roots can effectively promote the absorption of heavy metal lead in ryegrass and enhance plant resistance.Rhodococcus qingshengii-ryegrass combined remediation technology can be used to repair lead-contaminated soil,provide a new method for remediation of heavy metal pollution in soil,and provide a new idea for the development of microbial agents.
作者
焦郑同
朱琳
邹海明
王艳
JIAO Zhengtong;ZHU Lin;ZOU Haiming;WANG Yan(College of Resource and Environment,Anhui Science and Technology University,Fengyang 233100,China)
出处
《安徽科技学院学报》
2022年第1期32-37,共6页
Journal of Anhui Science and Technology University
基金
安徽省科技重大专项(202103b06020018)。
关键词
樊庆生红球菌
铅污染土壤
黑麦草
微生物-植物联合修复
Rhodococcus qingshengii
Lead soil pollution
Hyperaccumulator
Microbial-plant joint repair
