摘要
目的 了解广州地区献血人群CMV-IgG阳性率,探讨不同检测试剂检测效能的差异,为采供血系统提高血液安全,提升临床服务能力奠定基础。方法 随机抽取2020年7~10月在本中心献血且常规筛查合格的献血者血液标本630份,使用酶联免疫(ELISA)试剂(单试剂)检测CMV-IgG。其中180份标本使用2种酶免试剂以及1种电化学发光(ECLIA)试剂做平行检测,对检测结果为阴性的标本进行CMV-DNA定量检测,对不同试剂检测结果进行比较分析。结果 630份标本中共筛查出598份阳性。使用3试剂检测的180份标本中,2种ELISA试剂检测结果分别为171份阳性和175份阳性,ECLIA试剂检测结果为175份阳性,3种试剂的检测结果具有一致性(Kappa>0.4),阳性率差异无统计学意义。180份标本中有阴性标本11份,其中3试剂均阴性标本3份,双试剂阴性标本2份,单试剂阴性标本6份,单试剂阴性标本均为ELISA试剂阴性。11份阴性标本的CMV-DNA检测结果均为阴性。结论 广州地区献血人群的CMV-IgG阳性率为94.9%(598/630),提示CMV流行程度高,在向免疫力低下的患者提供血液制品时可以考虑筛选CMV血清阴性血液以提高受血者安全。ELISA试剂和ELCIA试剂对CMV-IgG的检测结果一致性较高,电化学发光试剂的检测效能优于酶免试剂。
Objective To investigate the CMV-IgG positive yeild among blood donors in Guangzhou and explore the differences in the efficacy of three test reagents, aimed at improving blood safety and service capacity of blood centers.Methods A total of 630 blood samples from eligible blood donors from July to October 2020 in our center were randomly selected and screened for CMV-IgG by one ELISA reagent.Among them, 180 samples were tested in parallel using three reagents(two ELISA reagents and one ECLIA reagent),and those tested negative were conducted quantitative CMV-DNA detection.The test results of different reagents were compared and analyzed.Results Out of the 630 samples, a total of 598 positive samples were screened out, including 180 samples yielded by three reagents, 171 and 175 by the two ELISA reagents, respectively, and 175 by ECLIA.The results given by three reagents were consistent(Kappa>0.4),and no significant difference in the positive yeild by three reagents was found.In the 180 samples, 11 were negative, among which 3,2 and 6 samples were negative by all three reagents, two reagents and one reagent(ELISA),respectively.All the 11 samples were tested negative for CMV-DNA.Conclusion The yeild of positive CMV-IgG in blood donors was 94.9%(598/630),suggesting a high prevalence of CMV in Guangzhou. CMV serologically negative blood should be considered when providing blood products to immunocompromised patients to improve the safety of recipients.The detection results of ELISA reagents and ECLIA reagent for CMV-IgG are consistent, but ECLIA reagent has better detection efficacy.
作者
单振刚
廖峭
许茹
王敏
黄杰庭
钟惠珊
付涌水
戎霞
SHAN Zhengang;LIAO Qiao;XU Ru;WANG Min;HUANG Jieting;ZHONG Huishan;FU Yongshui;RONG Xia(Guangzhou Blood Center,Guangzhou 510095,China)
出处
《中国输血杂志》
CAS
2022年第5期549-552,共4页
Chinese Journal of Blood Transfusion
关键词
献血人群
巨细胞病毒
抗体阳性率
blood donors
cytomegalovirus
antibody positive rate
