摘要
目的:探讨醛酮还原酶家族1成员C1(AKR1C1)在良性前列腺增生(BPH)细胞系中的功能。方法:运用siRNA以及过表达质粒干扰BPH-1及WPMY-1细胞系中的AKR1C1基因,采用实时荧光定量PCR及Western Blot进行基因敲减以及过表达之后的效率验证;借助MTT的方法测量不同处理组中细胞的增殖活性;采用实时荧光定量PCR、Western Blot和流式技术对不同的处理组中细胞的凋亡率、细胞的周期变化情况进行检测。结果:在BPH-1及WPMY-1细胞系中,AKR1C1基因的敲减使细胞增殖减慢,细胞凋亡率上升,而细胞周期无明显变化;AKR1C1基因的过表达使细胞增殖加快,细胞的凋亡率下降,细胞周期无明显影响。结论:AKR1C1在良性前列腺增生疾病中的高表达,可能与疾病的发生及进展相关,这可为寻找良性前列腺增生的治疗靶点提供线索。
Objective:To investigate the function of AKR1C1 in benign prostatic hyperplasia(BPH)cell lines.Methods:siRNAs and overexpressed plasmids were used to interfere AKR1C1 gene in BPH-1and WPMY-1 cell lines.The gene knockdown and overexpression efficiency were verified by real-time quantitative PCR and Western Blot.MTT method was used to measure the proliferation activity of cells in different treatment groups.The apoptosis rate and cell cycle changes in different treatment groups were detected by real-time fluorescence quantitative PCR,Western Blot,and flow cytometry.Results:In BPH-1 and WPMY-1 cell lines,AKR1C1 knockdown slowed cell proliferation and increased cell apoptosis rate,while cell cycle did not change significantly.The overexpression of AKR1C1 gene accelerated cell proliferation,decreased cell apoptosis rate,and had no significant effect on cell cycle.Conclusion:The high expression of AKR1C1 in BPH may be related to the occurrence and progression of the disease,which may provide clues for finding therapeutic targets of BPH.
作者
叶林朋
吕源
杨中华
YE Linpeng;LYU Yuan;YANG Zhonghua(Dept.of Urology,Huangmei People’s Hospital,Huanggang 435500,Hubei,China;Dept.of Urology,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei,China)
出处
《武汉大学学报（医学版）》
CAS
2022年第3期388-393,共6页
Medical Journal of Wuhan University
