摘要
为了解浙江四明山林地土壤微生物群落结构特征和多样性,探讨其与土壤肥力因子的关系,运用高通量测序技术,研究毛竹林、黄山松林、鹅掌楸林、樱花和红枫混交林4种典型林地,土壤微生物群落组成及多样性的变化。测序OTUs数量分析显示,黄山松林土壤的OTUs显著少于其他林地,特有OTUs也为最少;研究区域内,不同林地土壤细菌和真菌群落的Shannon指数和Simpson指数差异不显著,Ace指数和Chao 1指数则差异明显,其中黄山松林土壤微生物的Ace和Chao 1指数显著小于其他林地。土壤微生物群落在门水平上群落组成分析显示,土壤细菌优势菌门主要包括变形菌门、酸杆菌门、放线菌门和绿弯菌门,土壤真菌优势菌门主要包括子囊菌门、担子菌门和被孢菌门,不同林地之间土壤微生物群落丰度有明显差异。聚类分析显示各林地土壤微生物群落结构有较强的一致性和特异性。冗余分析结果显示,碱解氮、有效磷、速效钾、有机质等是影响区域林地土壤微生物群落结构多样性的主要因子。
To learn the structure characteristics and diversity of soil microbial community and its relationship with forest soil fertility factors in Siming Mountain area of Zhejiang Province,high-throughput sequencing technology was used to soil microbes with such 4 typical plantations as Phyllostachys edulis forest,Pinus taiwanensis forest,Liriodendron chinensis forest,and Prunus serrulata&Acer palmatum mixed forest.The OTUs quantity analysis showed that the OTUs with P.taiwanensis forest was significantly less than that of other plantations,as well as the least in the unique OTUs.The Shannon index and Simpson index of soil bacteria and fungi communities in different plantation showed no significantly different in the area,while the Ace index and Chao 1 index showed significantly different,especially significantly lower with P.taiwanensis forest.At the phylum level,soil microbial community composition analysis showed that the dominant phyla of soil bacteria mainly included Proteobacteria,Acidobacteria,Actinobacteria and Chloroflexi while the dominant fungi phyla mainly included Ascomycota,Basidiomycota and Mortierellomycota.There were significant differences in the abundance of soil microbial communities among plantations.Cluster analysis showed that soil microbial community structure of the forest types had the strong consistency and specificity.Redundancy analysis results showed that alkaline nitrogen,available phosphorus,available potassium,organic matter,acting as main environmental factors,affect soil microbial community structure diversity of forest types in Siming Mountain area.
作者
李东宾
许志斌
何立平
吴家森
李金朝
Li Dongbin;Xu Zhibin;He Liping;Wu Jiasen;Li Jinzhao(Ningbo Forest Farm, Ningbo 315440,China;School of Environmental and Resource Sciences,Zhejiang A&F University,Hangzhou,311300,China;Ningbo City College of Vocational Technology, Ningbo 315400, China)
出处
《江苏林业科技》
2022年第2期17-23,51,共8页
Journal of Jiangsu Forestry Science & Technology
基金
宁波市科学技术局公益类科技计划项目“四明山区域不同经营类型生态监测与对策研究”(2019C10027)。
关键词
林地
土壤微生物
群落结构
多样性
高通量测序
Forest land
Soil microbe
Community structure
Diversity
High-throughput sequencing
