摘要
盐碱胁迫是制约菜豆产业发展的重要因素之一,筛选菜豆幼苗期耐碱性鉴定指标对耐碱菜豆品种选育及促进盐碱地菜豆栽培具有重要意义。本试验以35个菜豆品种为试验材料,以60 mmol·L^(-1) NaHCO_(3)溶液模拟碱胁迫对菜豆幼苗进行处理,分别测定净光合速率、胞间CO_(2)浓度、气孔导度、丙二醛含量、株高、根长、地上部干/鲜质量、地下部干/鲜质量等共10个指标。通过主成分分析筛选菜豆幼苗期耐碱性鉴定指标,使用模糊隶属函数及聚类分析对菜豆苗期的耐碱性进行综合评价。结果表明,碱胁迫明显抑制菜豆幼苗生长,根长、地上部干/鲜质量、地下部干/鲜质量、净光合速率、胞间CO_(2)浓度及气孔导度等8个指标可作为快速鉴定菜豆幼苗期耐碱性的主要指标。通过对综合评价值(D值)排序及聚类分析,将35个品种分为耐碱性不同的5类,粉冠为耐碱品种,14-4-1-3等4个品种为相对耐碱品种,赤裕21号等13个品种为中等耐碱品种,44-1-1等16个品种为相对碱敏感品种,泰来白条为碱敏感品种。
Saline-alkali stress is one of the important factors restricting common bean industry development.Screening identification index for alkali tolerance of common bean in seedling stage has great importance in breeding alkali-tolerant common bean varieties and promoting common bean cultivation in saline-alkali land.This experiment took 35 common bean varieties as test materials,simulated 60mmol·L^(-1)NaHCO_(2)solution as alkali stress,and treated common bean seedlings.Ten indicators including net photosynthetic rate,intercellular CO_(2)concentration,stomatal conductance,malondialdehyde content,plant height,root length,and dry/fresh weight of the above-ground part and underground part were measured,respectively.The identification indicators for alkali tolerance of common bean in seedling stage were screened by principal component analysis,and alkali tolerance of common bean in seedling stage was comprehensively evaluated by fuzzy membership function and clustering analysis.The results showed that alkali stress could significantly inhibit the growth of common bean seeldings.Eight indicators including root length,dry/fresh weight of the above-ground part,dry/fresh weight of the underground part,net photosynthetic rate,intercellular CO_(2)concentration and stomatal conductance could be used as main indicators for rapid identifying alkali tolerance of common bean in seedling stage.These 35 varieties were divided into 5 types with different alkali resistance through sorting the comprehensive evaluation value(D value)and clustering analysis.‘Fenguan’was alkali-resitant variety.4 varieties such as‘14-4-1-3’were relative alkali-tolerant varieties.13 varieties such as‘Chiyu No.21’were medium alkali-tolerant varieties.16 varieties such as‘44-1-1’were relative alkali-sensitive varieties.‘Tailaibaitiao’was alkali-sensitive variety.
作者
苏艺
冯国军
刘大军
杨晓旭
闫志山
刘畅
SU Yi;FENG Guojun;LIU Dajun;YANG Xiaoxu;YAN Zhishan;LIU Chang(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin 150081,Heilongjiang,China)
出处
《中国蔬菜》
北大核心
2022年第6期16-24,共9页
China Vegetables
基金
黑龙江省省属高等学校基本科研业务费项目(2020-KYYWF-1026)。
关键词
菜豆
碱胁迫
鉴定指标
品种筛选
common bean
alkali stress
identification index
variety screening
