摘要
目的探讨鼠疫耶尔森菌Chro392基因的组成结构及生物学功能。方法本试验为预测采用生物信息学软件对该基因的理化性质、磷酸化位点、糖基化位点、亲/疏水性、跨膜区、保守结构域、信号肽、二级结构、以及T、B细胞抗原表位进行分析及预测。结果Chro392基因核酸序列长度723 bp,编码蛋白由240个氨基酸构成,理论等电点为6.84,分子式为C_(1212)H_(1927)N_(305)O_(373)S_(8),不含有跨膜区,在第1-37氨基酸位点含有信号肽,属于稳定的亲水性蛋白,共存在磷酸化位点27个,其中含量最高的磷酸化位点是苏氨酸。二级结构主要由延伸链和无规则卷曲构成,分别占29.17%和34.58%。该蛋白含有8个B细胞抗原表位。Chro392蛋白存在20个T细胞抗原表位,其中限制性Th细胞表位15个,限制性CTL细胞表位5个。结论生物信息学预测Chro392蛋白含有较多的B、T细胞抗原表位,具有良好的抗原性,可为鼠疫耶尔森菌的疫苗潜在候选抗原。
Objective To investigate the structure and biological function of Chro392 gene of Y.pestis.Methods This experiment was to predict the physicochemical properties,phosphorylation site,glycosylation site,hydrophilicity/hydrophobicity,transmembrane region,conserved domain,signal peptide,secondary structure,and T/B cell epitope of the gene using bioinformatics software to analysis and prediction.Results The nucleic acid sequence of Chro392 gene is 723 bp in length and contained 5 open reading frames.The encoded protein is composed of 240 amino acids,the theoretical isoelectric point is 6.84,and the molecular formula is C_(1212)H_(1927)N_(305)O_(373)S_(8).It does not contain a transmembrane region,and contains a signal peptide at amino acid positions 1 to 37,which is stable and hydrophilic proteins.There are 27 phosphorylation sites,and threonine is the phosphorylation site with the highest content.It is slightly more likely that the protein is localized to the cytoplasm.The secondary structure is mainly composed of extended chains and random coils,accounting for 29.17%and 34.58%,respectively.Epitope prediction shows that the protein contains 8 B cell epitopes,mainly located in 6-11,14-22,58-69,72-96,127-153,161-177,183-191,199-237.The amino acid residue at or near the position.There are 20 T cell epitopes in Chro392 protein,including 15 restricted Th cell epitopes and 5 restricted CTL cell epitopes.Conclusion Chro392 protein is predicted by bioinformatics to contain more B and T cell epitopes and has good antigenicity.It is speculated that this gene is expected to be used as a vaccine candidate antigen of Y.pestis.
作者
王姝懿
李建云
胡艳红
刘芳
于欣欣
武正华
赵学亮
WANG Shu-yi;LI Jian-yun;HU-Yan-hong;LIU fang;YU Xin-xin;WU Zheng-hua;ZHAO Xue-liang(Inner Mongolia Center for Disease Control and Prevention,Hohhot 010031,China;College of Veterinary Medicine,Northwest A&F University)
出处
《中国病原生物学杂志》
CSCD
北大核心
2022年第3期303-307,共5页
Journal of Pathogen Biology
基金
内蒙古自治区自然科学基金项目(No.2021BS08016)。
