摘要
对戟叶鹅绒藤地上部分和根的粉末及7种提取剂的提取液进行了红外光谱和紫外-可见光谱扫描.根据紫外-可见光谱特征,以70%乙醇为提取剂,以提取液在最大吸收波长304 nm处吸光度值为指标,通过正交试验优化了超声提取工艺.结果表明超声提取戟叶鹅绒藤地上部分物质的最佳工艺为:料液比1∶20(g∶mL),浸泡时间20 min,温度50℃,超声时间40 min.本研究关于戟叶鹅绒藤地上部分化学成分的超声提取工艺及总成分含量以紫外光谱进行检测的方法操作简便,结果稳定可靠,该方法的精密度、稳定性和重现性的相对标准偏差分别为0.01%~0.15%,0.29%~0.33%和0.13%~0.27%,说明方法良好.本研究为系统进行戟叶鹅绒藤的鉴定、质量控制及化学成分研究提供指导.
Cynanchum acutum L.subsp.sibiricum(Willdenow)K.H.Rechinger(abbreviated C.acutum subsp.sibiricum)asclepiadoideae Cynanchum Linn perennial twining vine,that has widely distributed in Xinjiang,Gansu,Ningxia and other places in China.All parts of the plant have medicinal value.But there were no reports of chemical composition and bioactivity of C.acutum subsp.sibiricum in China.In this study,the C.acutum subsp.sibiricum from Xinjiang of China was divided into the aerial parts and roots.Firstly,the infrared spectra(IR)of the aerial part and root powder were measured,the IR of the aerial part and the root are similar in shape,and the main absorption peaks were 3380,2930,1751,1643,1438,1338,1257,1105,1072,782,670 and 582 cm;.Secondly,the aerial part and root was ultrasound-assisted extracted with petroleum ether(60℃~90℃),chloroform,acetic ether,n-butyl alcohol,absolute ethyl alcohol,70%ethanol and water,respectively.Then the extracts were diluted and scanned 200~700 nm spectra.Results showed the different parts and different extract have different UV-Vis spectroscopy characteristics.The UV-Vis spectroscopy of the various solvent extracts of aerial parts,it was similar in 200~280 nm peak,the absorption peak of this range has no obvious characteristics.In 280~400 nm,petroleum ether extract has an inverted absorption peak at 294 nm,several other extract peaks in the range of 294~314 nm has a maximum absorption peak,the peak position and peak shape was very similar.In the visible region 400~700 nm,the organic solvent extracts had absorption peaks at near 414,430,450,472 and 666 nm.414 nm was the second absorption peak,and the 666 and 450 nm,represent the chlorophyll A and chlorophyll B absorption peak,respectively.However,the water extract of the aerial part has not chlorophyll absorption peaks and no other absorption peaks in 400~700 nm.The UV-Vis spectroscopy of the various solvent extracts of the root,there has a maximum absorption peak at about 300 nm,there has not chlorophyll absorption peaks and no other absorptio
作者
赵淑杰
张田田
姜斌
刘磊
李玉新
ZHAO Shu-jie;ZHANG Tian-tian;JIANG Bin;LIU Lei;LI Yu-xin(College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China;National Engineering Laboratory for Druggable Gene and Protein Screening,Northeast Normal University,Changchun 130024,China;College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
出处
《分子科学学报》
CAS
北大核心
2022年第1期54-60,共7页
Journal of Molecular Science
基金
国家自然科学基金资助项目(81502284)
吉林省科技发展计划项目(20150101188JC)
吉林省择优资助博士后科研基金资助项目(2015年度)。
关键词
戟叶鹅绒藤
红外光谱
紫外-可见光谱
超声提取
正交试验
Cynanchum acutum subsp.sibiricum
IR spectra
UV-Vis spectra
ultrasonic extraction
orthogonal test
