摘要
目的:探讨复方七芍降压片对原发性高血压左室肥厚大鼠的血管紧张素Ⅱ1型受体(AT1)介导的JAK/STAT信号通路影响。方法:11周龄原发性高血压大鼠随机分为模型组、复方七芍降压片组、缬沙坦组,以血压正常Wistar-Kyoto大鼠(WKY)作为对照组,每组15只。观察大鼠治疗前后的血压、血浆血管紧张素Ⅱ(AngⅡ)浓度、心肌组织AT1 mRNA表达、JAK/STAT信号通路相关蛋白表达的情况。结果:模型组、复方七芍降压片组、缬沙坦组大鼠给药前收缩压比较,差异无统计学意义(P>0.05),给药后复方七芍降压片组和缬沙坦治疗组大鼠收缩压较治疗前均有下降,与模型组大鼠比较,差异有统计学意义(P<0.01),给药后复方七芍降压片组和缬沙坦组同时间点比较,差异无统计学意义(P>0.05)。与对照组比较,模型组血浆AngⅡ水平、心肌组织AT1 mRNA均明显升高,差异均有统计学意义(P<0.01);复方七芍降压片组和缬沙坦组血浆AngⅡ水平、心肌组织AT1 mRNA均低于模型组,差异均有统计学意义(P<0.01);复方七芍降压片组与缬沙坦组比较,差异无统计学意义(P>0.05)。模型组Janus激酶(JAK1)、信号转导子及激活子(STAT3)、天冬氨酸特异性的半胱氨酸蛋白酶-3 (Caspases-3)高表达,复方七芍降压片组和缬沙坦组JAK1、STAT3、Caspase-3表达减少,与模型组比较,差异有统计学意义(P<0.05,P<0.01)。结论:复方七芍降压片可能是通过作用于AT1介导的JAK/STAT信号通路相关蛋白,抑制心肌细胞凋亡发挥逆转高血压左室肥厚的作用。
Objective:To investigate the effect of compound Qishao Jiangya tablets on JAK/STAT signal pathway mediated by angiotensin Ⅱ type 1 receptor AT1 in spontaneously hypertensive rats with left ventricular hypertrophy.Methods:11 week old spontaneously hypertensive rats were randomly divided into model group,compound Qishao Jiangya tablets group and valsartan group.Wistar Kyoto rats(WKY) with normal blood pressure were used as the control group,with15 rats in each group.The blood pressure,plasma angiotensin Ⅱ(Ang Ⅱ) concentration,AT1 mRNA expression and JAK/STAT signal pathway related protein expression were observed before and after treatment.Results:There was no significant difference in systolic blood pressure between model group,compound Qishao Jiangya tablets group and valsartan group before administration(P>0.05).After administration,the systolic blood pressure of compound Qishao Jiangya tablets group and valsartan treatment group decreased compared with that before treatment,and there was significant difference compared with model group(P<0.01).After administration,there was no significant difference between compound Qishao Jiangya tablet group and valsartan group at the same time point(P>0.05).Compared with the control group,the plasma Ang Ⅱ level and myocardial AT1 mRNA in the model group were significantly higher(P<0.01).The levels of plasma Ang Ⅱ and myocardial AT1 mRNA in compound Qishao Jiangya tablets group and valsartan group were significantly lower than those in model group(P<0.01).There was no significant difference between compound Qishao Jiangya tablet group and valsartan group(P>0.05).Janus kinase(JAK1),signal transducer and activator(STAT3) and cysteine aspartate specific protease-3(Caspase-3) were highly expressed in the model group,and the expression of JAK1,STAT3 and Caspase-3 in the compound Qishao Jiangya tablets group and valsartan treatment group decreased.Compared with the model group,the difference was statistically significant(P<0.05,P<0.01).Conclusion:Compound Qishao Jiangya t
作者
吴双华
王婷
罗勇
康永清
唐新桥
易添
王欣宇
WU Shuanghua;Wang Ting;LUO Yong;KANG Yongqing;TANG Xinqiao;YI Tian;WANG Xinyu
出处
《新中医》
CAS
2022年第9期6-10,共5页
New Chinese Medicine
基金
湖南省自然科学基金项目(2019JJ60082)
2021年度湖南省中医药科研计划项目(2021130)
2018年株洲市卫生人才135工程重点科研资助计划项目(2019JJ60082)。
