摘要
基于CRISPR/Cas系统识别和切割靶标DNA或RNA的特性不仅开发出精准、高效的基因编辑技术,也设计出革新的高灵活性和灵敏度的病原体检测技术。该文介绍并阐明CRISPR/Cas系统的不同作用机制和最新分类,着重综述“2”类CRISPR系统在诸多病原体检测中的研究进展,讨论分析了CRISPR/Cas系统在病原体检测领域存在的优点以及面临的挑战。
CRISPR/Cas systems have been harnessed gene editing,based on their ability to accurately recognize and cleave specific DNA or RNA sequences.Following recognition of the target sequence,moreover,couple CRISPR/Cas systems with nucleic acid amplification or lateral flow systems also can be employed for pathogen detection with accurate and highly sensitive trait.In this review,the CRISPR/Cas system is introduced,and next different mechanism and the latest classification of the system are expounded,meanwhile,the research achievements of "2" CRISPR system in the detection of many pathogens is highlighted.Furthermore,current challenges and future perspectives of CRISPR-based pathogen detection are discussed.
作者
冯万有
蒙丽娜
陈春
何立珍
覃广胜
石德顺
FENG Wan-you;MENG Li-na;CHEN Chun;HE Li-zhen;QIN Guang-sheng;SHI De-shun(School of Environment and Life Science,Nanning Normal University,Nanning 530001,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi University,Nanning 530005,China;Guangxi Institute for Food and Drug Control,Nanning 530021,China;Buffalo Research Institute,Chinese Academy of Agricultural Science,Nanning 530001,China)
出处
《生物技术》
CAS
2022年第2期258-267,251,共11页
Biotechnology
基金
广西自然科学基金项目(2017GXNSFBA198170)
广西中青年教师基础能力提升项目(62050)
中国农业科学院水牛研究所创新实践基地合作研究项目(SNBC-2017-01)
南宁师范大学2020年国家级大学生创新创业训练计划项目(202010603020)。
