摘要
目的:完善明目十六味丸的质量标准。方法:对其形状及显微鉴别进行修订及规范化描述;采用薄层色谱法对明目十六味丸中决明子、当归、枸杞等进行定性鉴别研究;采用高效液相法,色谱柱为PhenomenexLuna C_(18)(250 mm×4.6 mm,5μm),以甲醇-乙腈-0.7%磷酸溶液(用三乙胺调pH值为6.0)(15∶1∶84)为流动相,流速1.0 mL/min,检测波长403 nm,柱温30℃,进行红花中羟基红花黄色素A含量测定。结果:决明子、当归和枸杞的薄层色谱主斑点清晰,分离良好,阴性对照无干扰;羟基红花黄色素A在79.1~1185.9 ng(r=0.9999)范围内线性良好,平均加样回收率为98.6%,RSD为0.49%。结论:建立的TLC和HPLC专属性强、重复性好、准确可靠,能够有效评价和控制蒙成药明目十六味丸质量。
Objective:To improve the quality standard of"Mingmu Liuwei Pills".Methods:The shape and microscopic identification were revised and standardized description;the qualitative identification of cassia seed,angelica and wolfberry in Mingmu Shiliuwei Pills was carried out by thin-layer chromatography;high performance liquid chromatography was used,and the chromatographic column was PhenomenexLuna C_(18)(250 mm×4.6 mm,5μm),methanol-acetonitrile-0.7%phosphoric acid solution(adjusted to pH 6.0 with triethylamine)(15∶1∶84)as mobile phase,flow rate 1.0 m L/min,detection The wavelength of 403 nm and the column temperature of 30℃were used to determine the content of hydroxysafflor yellow A in safflower.Results:The main spots of TLC of Cassia,Angelica and Lycium barbarum were clear and well separated,and the negative control had no interference;hydroxysafflor yellow A had good linearity in the range of 79.1~1185.9 ng(r=0.9999),and the average sample recovery was 98.6%with an RSD of 0.49%.Conclusion:The established TLC and HPLC methods have strong specificity,good reproducibility,accuracy and reliability,and can effectively evaluate and control the quality of Mongolian medicine Mingmu Shiliuwei Pills.
作者
娜仁图雅
包顺茹
韩塔娜
籍学伟
王栋
Narentuya;Baoshunru;Hantana;JI Xue-wei;WANG Dong(Inner Mongolian Institute for Drug Control,Hohhot 010000,China)
出处
《中国民族医药杂志》
2022年第5期55-58,共4页
Journal of Medicine and Pharmacy of Chinese Minorities
关键词
明目十六味丸
质量标准研究
羟基红花黄色素A
薄层色谱
高效液相色谱
Mingmu Shiliuwei pills
quality standard research
hydroxysafflor yellow A
thin layer chromatography
high performance liquid chromatography
