摘要
为探究微纳米曝气对水库沉积物细菌及磷功能菌群落的影响,以三明市东牙溪水库为研究对象,通过Illumina高通量测序,分析了曝气前后沉积物细菌、聚磷菌与有机解磷菌的群落组成、结构及其与环境因子的相关性。结果表明,微纳米曝气对底部产生充氧作用,进而对沉积物微生物的群落造成一定影响,微纳米曝气后,变形菌门(Proteobacteria)的相对丰度上升了24.7%,厚壁菌门(Firmicutes)上升了8.3%,而绿弯菌门(Chloroflexi)下降了5.9%,酸杆菌门(Acidobacteria)下降了7.0%。曝气前,沉积物中聚磷菌的优势菌属包括Syntrophus(26.79%)、Anaeromyxobacter(14.6%)、Nitrospira(12.6%)和Anaerolinea(11.3%);而曝气后,聚磷菌优势菌属则为Pseudomonas(65.1%)和Clostridium(22.9%)。有机解磷菌的优势菌属在微纳米曝气前后同样发生了改变:在曝气前,优势菌属为Azospira、Mesorhizobium、Pseudomonas、Chelatococcus、Variovorax,分别占8.6%、5.4%、5.3%、5.2%、和5.2%;在曝气后,优势菌属则为Azospira、Variovorax、Chelatococcus、Pseudomonas、Acidovorax,分别占11.3%、6.5%、6.1%、5.9%和5.8%。冗余分析结果表明,不管是聚磷菌还是有机解磷菌,其群落组成与沉积物表层的溶解氧(DO)均显著相关(P<0.05)。微纳米曝气工程的实施,对水库沉积物微生物的群落组成、结构有一定影响。
In order to explore the effects of micro-nanometer aeration on bacterial and phosphorus functional bacterial communities in reservoir sediments, Dongyaxi reservoir in Sanming city was taken as the research object in this study. Illumina high-throughput sequencing was used to analyze the community composition and structure of bacteria, phosphorus-accumulating bacteria and organic phosphorus solubilizing bacteria before and after micro-nanometer aeration, as well as the correlation with environmental factors. The results show that micro-nanometer aeration had a certain influence on the microbial community in the sediment through oxygenation. After aeration, the abundance of Proteobacteria and Firmicutes increased by 24.7% and 8.3%,respectively. The abundance of Chloroflexi and Acidobacteria decreased by 5.9% and 7.0%, respectively. Before aeration, the dominant genera of phosphorus-accumulating bacteria in sediments included: Syntrophus(26.79%),Anaeromyxobacter(14.6%), Nitrospira(12.6%) and Anaerolinea(11.3%). After aeration, the dominant species were Pseudomonas(65.1%) and Clostridium(22.9%). The dominant genera of organic phosphorus solubilizing bacteria also changed before and after micro-nanometer aeration. Before micro-nanometer aeration, the dominant genera were Azospira, Mesorhizobium, Pseudomonas, Chelatococcus and Variovorax, accounting for8.6%, 5.4%, 5.3%, 5.2% and 5.2%, respectively. After micro-nanometer aeration, the dominant genera were Azospira, Variovorax, Chelatococcus, Pseudomonas and Acidovorax, accounting for 11.3%, 6.5%, 6.1%, 5.9%and 5.8%, respectively. The results of redundancy analysis showed that there was a significant correlation between the community composition and the dissolved oxygen(DO) in sediment surface layer(P<0.05),regardless of phosphorus-accumulating bacteria or organic phosphorus solubilizing bacteria. The implementation of micro-nanometer aeration project has a certain influence on the composition and structure of microbial community in reservoir sediment.
作者
刘彩凤
佘晨兴
苏玉萍
张太阳女
黄磊
林志蓉
陈文婷
林映津
LIU Caifeng;SHE Chenxing;SU Yuping;ZHANG Taiyangnv;HUANG Lei;LIN Zhirong;CHEN Wenting;LIN Yingjin(College of Environmental Science and Engineering,Fujian Normal University,Fuzhou 350007,China;Fujian Key Laboratory of Pollution Control and Resource Reuse,Fuzhou 350007,China;Fujian Huachuan Technology Co.Ltd.,Fuzhou 350007,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2022年第4期1340-1352,共13页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金资助项目(41573075)
福建省自然科学基金资助项目(2021J01192,2018J01742)
高校产学研合作项目(SC-292,DH-1558,21NB000922)。
关键词
水库沉积物
微纳米曝气
微生物群落
磷功能菌
reservoir sediment
micro-nanometer aeration
microbial community
phosphorus functional bacteria
