摘要
中药具有丰富的化学成分和复杂的作用途径,在治疗和预防疾病方面疗效显著,为保证中药质量及开发新药,一些分析检测方法应运而生。基质辅助激光解吸电离质谱法(MALDI-MS)是一种“软电离”的新型质谱技术,具有高通量、高灵敏度、低成本、低样品消耗量、线性范围宽、电离温和、适用于热不稳定物质等特点,为中药的分子水平研究提供了技术支撑。目前该技术被广泛用于中药成分分析和组学研究领域,在药材真伪鉴别、分子实时筛选和构建活性成分的代谢网络途径方面发挥重要作用,其中,选择合适的基质和样品制备技术是保证检测效果的关键。随着新型基质的开发与优化、样品制备技术的不断完善、MALDI-MS与多种分析方法的联用,将会大幅提高其检测效果。基于此,笔者概述了MALDI-MS在中药领域的应用情况,包括高通量检测中药活性成分、监测机体内药物及其代谢物状况、原位可视化表征中药活性成分组织分布信息等,并探讨了MALDI-MS的应用前景和不足之处,以期为中药成分鉴定、药物利用及代谢的相关研究提供技术支撑。
Traditional Chinese medicine(TCM),which owns abundant chemical components and complex action pathways,has been widely recognized in the prevention and treatment of diseases. Some analysis methods have been emerged in order to ensure the quality of TCM and to develop new TCM drugs. Matrixassisted laser desorption ionization mass spectrometry(MALDI-MS)is a soft ionization mass spectrometric technique with the advantages of high throughput,high sensitivity,low cost and so on. It provides technical support for the molecular level study on TCM. At present, this technique has been used in the field of composition analysis and metabonomics research of TCM,and plays an important role in the identification of Chinese herbal medicines,real-time molecular screening and the construction of metabolic network pathway of active ingredients. Among them,the selection of appropriate matrix and sample preparation technology is the key to ensure the detection effect of MALDI-MS. With the development and optimization of new matrix,the continuous improvement of sample preparation technology and the combination of MALDI-MS with various analytical methods will greatly improve the detection effect. Based on this,this paper discusses the application of MALDI-MS in TCM,including high-throughput detection of active ingredients in TCM,monitoring of the original medicines and their metabolites in vivo, and in situ visualization and characterization of tissue distribution information of active ingredients in TCM. It also discusses the application prospect and existing problems of MALDI-MS in TCM,so as to provide technical support for the identification of active ingredients in TCM,drug utilization and metabolism.
作者
张冠华
刘小莉
黄璐琦
王晓
刘伟
孙成龙
张华敏
马春霞
ZHANG Guan-hua;LIU Xiao-li;HUANG Lu-qi;WANG Xiao;LIU Wei;SUN Cheng-long;ZHANG Hua-min;MA Chun-xia(State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Institute of Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences,Beijing 100700,China;Postdoctoral Management Office,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第12期247-255,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央本级重大增减支项目(2060302)。
关键词
基质辅助激光解吸电离质谱法(MALDI-MS)
中药
活性成分
代谢物
质谱成像
原位可视化
组织分布
matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS)
traditional Chinese medicine
active ingredients
metabolites
mass spectrometry imaging
in situ visualization
tissue distribution
