摘要
Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109.Methods:The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry(GC-MS).The anti-proliferative,anti-migrative,and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed.Moreover,the expression of proteins associated with cell cycle,metastasis,and apoptosis was determined.Results:GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes.Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC_(50) values of(24.29±1.49),(19.16±2.27)and(6.97±0.86)μg/mL at 12,24,and 48 h,respectively.The expression levels of target proteins in the cell cycle(phase G_(1)/S),including cyclin D1,p21,and p53,were affected by Saussurea costus essential oil.The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2.Moreover,it induced apoptosis of Eca109 cells through the mitochondrial pathway,as well as inhibition of STAT3 phosphorylation.Conclusions:The essential oil from Saussurea costus exhibited anti-proliferative,anti-migrative,and apoptotic effects on Eca109 cells,and could be further explored as a potential anti-esophageal cancer agent.
基金
financially supported by the Scientific Research and Innovation Fund of Wuhan Asia General Hospital (Project No. WAGH-DSE-2022-005)
2020 General Planning Fund Project for Humanities and Social Sciences of the Ministry of Education,China (Project No. 20YJA880053)
Key Research Project of Philosophy and Social Sciences of Hubei Provincial Department of Education in 2020 (Project No. 20D026)
WUST National Defence Pre-research Foundation,China (Project No. GF202003)
Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology (Project No. OHIC2020G05)
National Undergraduate Innovation and Entrepreneurship Training Project (Project No. JCX201976)
