摘要
目的:观察温脾汤对脓毒症大鼠肠黏膜屏障功能的保护作用及其机制。方法:50只大鼠称重后按阿拉伯数字编号分为模型组、假手术组、中药低剂量组、中药中剂量组、中药高剂量组,每组各10只。中药低剂量组用温脾汤5.3g/(kg·d),中剂量组用温脾汤10.6g/(kg·d),高剂量组用温脾汤21.6g/(kg·d)。酶联免疫吸附测定法(ELISA)分析法测定血清D-乳酸、IL-17水平和肠道上皮细胞sIgA蛋白表达,备流式细胞实验检测Treg、Th17细胞,并进行组间比较分析。结果:相较于模型组,中药高剂量组、中剂量组、低剂量组血清D-乳酸、IL-17水平均更低(P<0.05)。相较于模型组,各组sIgA蛋白表达差异无统计学意义(P>0.05),但各用药组均有所降低。相较于模型组,中药低、中、高剂量组的Treg和Th17细胞含量均更高(P<0.05),且中药低、中、高剂量组之间呈明显上升趋势。结论:温脾汤对脓毒症大鼠肠黏膜屏障可起到保护作用,其机制可能与抑制炎症因子表达,调节免疫系统有关。
Objective:To observe the protective effect and mechanism of Warming Spleen Decoction on intestinal mucosal barrier function in sepsis rats.Methods:After weighing,50 rats were divided into model group,sham operation group,low-dose group of traditional Chinese medicine,middle-dose group of traditional Chinese medicine,and high-dose group of traditional Chinese medicine according to the Arabic numerals,with 10 rats in each group.The low-dose group of traditional Chinese medicine were given 5.3g/(kg·d)Warming Spleen Decoction,the middle-dose group given 10.6 g/(kg·d)Warming Spleen Decoction,and the high-dose group given 21.6 g/(kg·d)Warming Spleen Decoction.Enzyme-linked immunosorbent assay(ELISA)was used to determine serum D-lactic acid and IL-17 levels and the expression of sIgA protein in intestinal epithelial cells.Flow cytometry was used to detect Treg and Th17 cells,and the comparison between groups was analyzed.Results:Compared with the model group,the serum levels of D-lactic acid and IL-17 in the highdose,medium-dose,and low-dose groups of traditional Chinese medicine were lower(P<0.05).Compared with the model group,there was no significant difference in the expression of sIgA protein among the groups(P>0.05),but the drug groups were all decreased.Compared with the model group,the contents of Treg and Th17 cells in the low-dose,medium-dose and high-dose groups of traditional Chinese medicine were higher(P<0.05),and there was a significant upward trend among the low-dose,medium-dose and high-dose groups of traditional Chinese medicine.Conclusion:Warming Spleen Decoction can protect the intestinal mucosal barrier in sepsis rats,and its mechanism may be related to inhibiting the expression of inflammatory factors and regulating the immune system.
出处
《实用中医药杂志》
2022年第5期709-711,共3页
Journal of Practical Traditional Chinese Medicine
关键词
大鼠
温脾汤
肠黏膜屏障功能
保护
作用机制
Rats
Warming Spleen Decoction
Intestinal mucosal barrier function
Protection
Mechanism of action
