摘要
目的构建体外分区囊袋模型,探讨不同类型360°连续直角边缘人工晶状体(IOL)对晶状体上皮细胞(LECs)迁移的抑制作用。方法使用Transwell小室、细胞爬片、人源Ⅳ型胶原、IOL建立后发性白内障(PCO)体外分区囊袋模型作为模型组,并设置一个空白处理的Transwell小室作为对照组。人LECs细胞系SRA01/04培养于各组Transwell小室中,应用倒置显微镜观察各组中PCO早期病理表现;采用苏木精-伊红染色观察各组细胞形态改变。根据体外分区囊袋模型植入的IOL类型分为平台板式袢HydroSmart组、C型袢HydroSmart组、C型补偿袢Hydrophobic组,并设置不植入IOL为空白对照组。应用Transwell法检测各组IOL前囊膜区迁移细胞数目并计算前囊膜细胞迁移抑制率;应用细胞排斥区分析法检测各组IOL后囊膜区细胞迁移数目并计算后囊膜细胞迁移抑制率。结果模型组细胞培养48 h后体外分区囊袋模型后囊膜区可见细胞形成类似早期Soemmering环和小型Elschnig珍珠样小体等PCO特征性病理表现;苏木精-伊红染色显示模型组迁移的细胞呈纤维状。对照组中均未见类似细胞分布及形态改变。平台板式袢HydroSmart组、C型袢HydroSmart组、C型补偿袢Hydrophobic组前囊膜区域单位面积迁移细胞计数分别为18.80±5.53、24.67±9.80和34.47±10.80,后囊膜袢光学部移行区迁移细胞计数分别为56.43±9.00、162.20±16.38和121.30±12.01,IOL前囊膜细胞迁移抑制率分别为(92.02±1.94)%、(89.76±3.10)%和(86.27±4.54)%;后囊膜细胞迁移抑制率分别为(91.60±3.65)%、(70.14±5.35)%和(78.43±3.48)%。平台板式袢HydroSmart组前囊膜区迁移细胞数目明显少于C型补偿袢Hydrophobic组,细胞迁移抑制率明显高于C型补偿袢Hydrophobic组,差异均有统计学意义(均P<0.05);平台板式袢HydroSmart组后囊膜袢光学部移行区迁移细胞数、细胞迁移抑制率明显高于C型袢HydroSmart组和C型补偿袢Hydrophobic组,差异�
Objective To establish an in vitro capsular bag model and compare the inhibitory effects of different 360°square-edge intraocular lens(IOL)on lens epithelial cells(LECs)migration.Methods In vitro capsular bag model with posterior capsule opacification(PCO)was established using Transwell compartment,cell climbing slices,human collagen typeⅣ,and IOL.The models were divided into Plate-loop HydroSmart group,C-loop HydroSmart group,and C-compensation-loop Hydrophobic group according to the different square-edge IOL implanted.A blank control group was set using the Transwell compartment without IOL.The early PCO pathological manifestations in lens epithelial cell line SRA01/04 cultured in the Transwell compartment were observed with an inverted microscope.The cell morphology in different groups was observed by hematoxylin and eosin staining.The cell counting and cell migration inhibition rate of anterior capsule and posterior capsule were calculated by Transwell assay and cell-exclusion zone assay,respectively.Results The early pathological characteristics of PCO,such as early Soemmering ring and small Elschnig pearl,could be found in cells in the in vitro capsular bag model after 48-hour culture.The migrating cells in model groups were fibrous.No changes mentioned above were found in blank control group.The number of migrating cells in the anterior capsule of Plate-loop HydroSmart group,C-loop HydroSmart group,C-compensation-loop Hydrophobic group was 18.80±5.53,24.67±9.80,and 34.47±10.80,respectively,and the number of migrating cells in the optical area of the posterior capsule of the three groups was 56.43±9.00,162.20±16.38,and 121.30±12.01,respectively.The cell migration inhibition rate in the anterior capsule of Plate-loop HydroSmart group,C-loop HydroSmart group,C-compensation-loop Hydrophobic group was(92.02±1.94)%,(89.76±3.10)%,(86.27±4.54)%,respectively,and the cell migration inhibition rate in optical area of the posterior capsule of the three groups was(91.60±3.65)%,(70.14±5.35)%,(78.43±3.48)%
作者
廉飞玥
李阳
姜凌峰
沈皓月
赵江月
严肖啸
于佳明
秦宇
Lian Feiyue;Li Yang;Jiang Lingfeng;Shen Haoyue;Zhao Jiangyue;Yan Xiaoxiao;Yu Jiaming;Qin Yu(Department of Ophthalmology,the Fourth Affiliated Hospital of China Medical University,Key Lens Research Laboratory of Liaoning Province,Shenyang 110005,China)
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2022年第5期395-402,共8页
Chinese Journal Of Experimental Ophthalmology
基金
国家自然科学基金青年基金项目(81600717)
辽宁省自然科学基金项目(201602851、2021-MS-200)
"双一流"国家自然科学基金培育项目(114-3111210504)。
关键词
白内障
后囊膜混浊
细胞迁移抑制
人工晶状体
晶状体上皮细胞
体外囊袋模型
Cataract
Capsule opacification
Cell migration inhibition
Lenses,intraocular
Lens epithelial cells
In vitro capsular bag model
