摘要
Mung bean(Vigna radiata)sprouts are widely consumed worldwide due to their high nutritional value.However,the low yield and microbial contamination of mung bean sprouts seriously reduces their economic value.This study investigates the effects of non-thermal plasma on the quality and microbial reduction of mung bean sprouts by pretreatment of seeds in water for different times(0,1,3 and 6 min).The quality results showed that short-time plasma treatment(1 and 3 min)promoted seed germination and seedling growth,whereas long-time plasma treatment(6 min)had inhibitory effects.Plasma also had a similar dose effects on the total flavonoid and phenolic contents of mung bean sprouts.The microbiological results showed that plasma treatment achieved a reduction of native microorganisms ranging from 0.54 to 7.09 log for fungi and 0.29 to 6.80 log for bacteria at 96 h incubation.Meanwhile,plasma treatment could also efficiently inactivate artificially inoculated Salmonella typhimurium(1.83–6.22 log)and yeast(0.53–3.19 log)on mung bean seeds.The results of seed coat permeability tests and scanning electron microscopy showed that plasma could damage the seed coat structure,consequently increasing the electrical conductivity of mung bean seeds.The physicochemical analysis of plasma-treated water showed that plasma generated various long-and short-lived active species[nitric oxide radicals(NO·),hydroxyl radicals(·OH),singlet oxygen(1O2),hydrogen peroxide(H_(2)O_(2)),nitrate(NO_(3)^(-)),and nitrite(NO_(2)^(-))]in water,thus the oxidizability,acidity and conductivity of plasma-treated water were all increased in a treatment timedependent manner.The result for mimicked chemical mixtures confirmed the synergistic effect of activity of H_(2)O_(2),NO_(3)^(-)and NO_(2)^(-)on bacterial inactivation and plant growth promotion.Taken together,these results imply that plasma pretreatment of mung bean seeds in water with moderate oxidizability and acidity is an effective method to improve the yield of mung bean sprouts and reduce micro
作者
Dongjie CUI
Xiaoxia HU
Yue YIN
Yupan ZHU
Jie ZHUANG
Xiaojie WANG
Ruonan MA
Zhen JIAO
崔东洁;胡小霞;阴悦;朱育攀;庄杰;王小洁;马若男;焦浈(Henan Key Laboratory of Ion-Beam Bioengineering,School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450052,People's Republic of China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,People's Republic of China;School of Life Sciences and Basic Medicine,Xinxiang University,Xinxiang 453003,People's Republic of China)
基金
supported by National Natural Science Foundation of China(Nos.11605159 and 11405147)
Chinese Postdoctoral Science Foundation(No.2017M612412)
the Foundation of Key Technology Research Project of Henan Province(No.182102311115)
Key Discipline Construction Project of Zhengzhou University(No.32410257)
Youth Innovation Project of Key Discipline of Zhengzhou University(No.XKZDQN202002)
Natural Science Foundation of Henan Province(No.202300410013)。
