摘要
目的探讨长链非编码RNA(lncRNA)LINC00606对胰腺癌细胞增殖、侵袭及迁移的影响。方法(1)利用转录组测序技术检测3组胰腺癌组织与癌旁组织中的lncRNA表达水平,经对比分析筛选出目标lncRNA LINC00606。(2)采用PCR检测LINC00606在3种胰腺癌细胞系(BxPC-3、PANC-1、SW1990)和正常胰腺导管上皮细胞系(HPDE6c-7)中的表达。(3)通过oeRNA过表达质粒上调PANC-1细胞中LINC00606的表达水平,然后将其分为未转染组(Ctrl)、转染pcDNA3.1空载体组(Vector组)和转染重组质粒pcDNA3.1组(oe-LINC 00606);下调BxPC-3细胞中LINC00606水平,然后将其分为未转染组(Ctrl组)、转染空白(shNC组)及转染组(sh-LINC 00606组);采用RT-qPCR检测各组细胞中LINC00606的表达水平;运用平板克隆实验检测细胞克隆形成数,细胞划痕实验检测划痕愈合相对百分比,Transwell实验研究检测细胞侵袭迁移能力。结果(1)胰腺癌组织中LINC00606的表达显著降低(P<0.05);(2)胰腺癌细胞中LINC00606表达水平显著降低(P<0.05);(3)与Vector组相比,oe-LINC 00606组细胞克隆形成数、划痕愈合相对百分比、细胞迁移和侵袭数量降低(均P<0.05);与shNC组相比,sh-LINC00606组细胞克隆形成数、划痕愈合相对百分比、细胞迁移和侵袭数升高(P<0.05)。结论LINC00606低表达可促进胰腺癌细胞的增殖、侵袭及迁移能力,在胰腺癌发生发展过程中可能起着重要的调节作用。
objective To investigate the effects of long non-coding RNA(lncRNA)LINC00606 on the migration,proliferation and invasion of pancreatic cancer cells.Methods(1)The expression level of lncRNA in different types of sample tissues was detected by transcriptome sequencing,and the target RNA LINC00606 was determined by comparative analysis.(2)RT-qPCR were applied to detect expression of LINC00606 in three kinds of pancreatic cancer cells(BxPC-3,PANC-1,SW1990)and normal pancreatic ductal epithelial cell(HPDE6c-7).(3)The expression level of LINC00606 in PANC-1 cells was up-regulated by oeRNA overexpression plasmid,and then divided into untransfected group(Ctrl),transfected pcDNA3.1 empty vector group(Vector)and transfected recombinant plasmid pcDNA3.1 group(oe-LINC 00606);down-regulated the level of LINC00606 in BxPC-3 cells,and then divided them into untransfected group(Ctrl),transfected blank(shNC)and transfected group(shLINC 00606);The expression level of LINC00606 in each group was determined by RT-qPCR,Colony formation assay was used to detect the number of cell clones,wound-healing assay was used to detect the relative percentage of scratch healing,and the Transwell assay was used to detect the ability of cell invasion and migration.Results(1)The LINC00606 expression levels were significantly decreased in pancreatic cancer tissues.(2)PCR assay showed that the expression levels of the LINC00606 in pancreatic cancer cells were significantly lower than those in normal pancreatic cancer cells(P<0.05).(3)Compared with the Vetor group,colony formation,relative percentage of scratch healing,cell migration and invasion number,reduced in the oe-LINC 00606 group(P<0.05).Compared with the shNC group,colony formation,relative percentage of scratch healing,cell migration and invasion number,increased in the oe-LINC 00606 group(P<0.05).Conclusion In pancreatic cancer tissues,the low expression of LINC00606 promotes the proliferation,migration,and invasion of pancreatic cancer cells.It can be inferred that LINC00606 may play an im
作者
王哲近
李晨
麻忠武
宋洪亮
俞海波
WANG Zhejin;LI Chen;MA Zhongwu;SONG Hongliang;YU Haibo(Department of Hepatobiliary Surgery,Wenzhou Central Hospital/Dingli Clinical College of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China)
出处
《肝胆胰外科杂志》
CAS
2022年第5期288-294,共7页
Journal of Hepatopancreatobiliary Surgery
基金
浙江省基础公益研究计划项目(LGF19H160020)
温州市基础性科研项目(Y20180227L)。
