摘要
目的观察痰火方对脑缺血模型大鼠Caspase-3、胶质纤维酸性蛋白(GFAP)表达及血管新生的影响。方法将大鼠按随机数字表法分为假手术组、模型组、痰火方低剂量组、痰火方中剂量组、痰火方高剂量组、金纳多组。除假手术组外, 其余各组采用线栓法建立大鼠中动脉栓塞模型。造模2 h后, 痰火方低、中、高剂量组分别灌胃0.92、1.84、3.68 g/kg痰火方干浸膏粉溶液, 金纳多组灌胃金纳多60 mg/kg, 每24 h给药1次, 连续给药3 d。假手术组和模型组大鼠灌胃等体积生理盐水。采用四肢对称性评分评价肢体功能症状, 采用免疫荧光染色法检测梗死灶周围组织GFAP、Caspase-3蛋白表达, 评价大鼠星形胶质细胞活化、神经细胞凋亡情况, 采用免疫荧光双标法检测血小板-内皮细胞黏附分子(CD31)/硫酸软骨素蛋白多糖2(NG2)蛋白表达, 观察各组大鼠血管新生情况。结果与模型组比较, 术后48、72 h, 痰火方高剂量组大鼠四肢对称运动评分提高(P<0.01或P<0.05), 梗死灶周围皮层、纹状体Caspase-3阳性细胞数[皮层:(765.0±122.4)比(1 131.0±392.9)、纹状体:(895.9±389.8)比(1 401.9±453.1)]减少(P<0.05或P<0.01), CD31+/NG2+阳性细胞数[皮层:(1 355.0±257.9)比(825.4±308.1);纹状体:(1 290.9±400.9)比(675.2±259.7)]增多(P<0.01);梗死周围皮层GFAP阳性积分光密度[(4 210.00±1 226.38)比(7 935.78±2 001.98)]降低(P<0.01)。结论痰火方可减轻脑缺血大鼠肢体功能症状, 抑制星形胶质细胞活化, 下调Caspase-3表达, 促进血管新生。
Objective To study the effect of Tanhuo Formula(THW)on the expression of Glial Fibrillary Acidic Protein(GFAP),Caspase-3 and angiogenesis.Methods Rats were divided into sham group,model group,low-dose THW group,medium-dose THW group,high-dose THW group and Ginaton group according to random number table method.Except the sham group,rats in other groups were subjected to the middle cerebral artery occlusion via a suture method.After 2 hours,rats in the low,medium and high dose of THW groups were gavaged with 0.92,1.84 and 3.68 g/kg of THW dry extract powder solution respectively,and the Ginaton group were gavaged with 60 mg/kg of Ginaton,once every 24 hours for 3 days.Rats in sham operation group and model group were given equal volume of normal saline by gavage.The limb symmetry score was used to evaluate limb dysfunctions.The immunofuorescence staining of GFAP and Caspase-3 were applied to assess astrocyte activation and neuronal apoptosis,respectively.The double-labeled immunofluorescence staining of platelet-endothelial cell adhesion molecule(CD31)and chondroitinsulphate peoteoglycan(NG2)were performed to detect angiogenesis.Results Compared with the model group,rats in the high-dose of THW group showed increased limb symmetry score(P<0.01 or P<0.05),increased number of Caspase-3(cortex:765.0+122.4 vs.1131.0+392.9;striatum:895.9+389.8 vs,1401.9+453.1](P<0.01 or P<0.05]and CD31*/NG2*(cortex:1355.0+257.9 vs.825.4+308.1;striatum:1290.9+400.9 vs.675.2+259.7](P<0.01)positive cells in the perinfarct cortex and striatum,and attenuated the integrated optical density of GFAP in the perilesional cortex(4210.00+1226.38 vs.7935.78+2001.98)(P<0.01).Conclusions THW could ameliorate the limb functional disorders,inhibit astroglia activation,down-regulate the expression of Caspase-3,and enhanced angiogenesis in MCAO rats.
作者
李慢中
李明聪
赵婷
赵晖
冯雪枫
陆允
杨乐
詹宇
高利
Li Manzhong;Li Mingcong;Zhao Ting;Zhao Hui;Feng Xuefeng;Lu Yun;Yang Le;Zhan Yu;Gao Li(Department of Pharmacy,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;SchooI of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Department of Neurology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)
出处
《国际中医中药杂志》
2022年第3期292-297,共6页
International Journal of Traditional Chinese Medicine
基金
北京市科学技术委员会“十病十药”研发专项(Z171100001717012)。
关键词
脑缺血
痰火方
细胞凋亡
血管新生
大鼠
Brain ischemia
Tanhuo Formula
Apoptosis
Angiogenesis
Rats
