摘要
背景:细胞外囊泡可以通过参与细胞间信号免疫调节来控制炎症反应,同时也可修复牙周组织,促进牙周组织的再生。将该技术应用于慢性牙周炎的预防诊断与组织再生,有望成为一种无细胞组织再生方式。目的:就细胞外囊泡的合成与分泌、生物学功能、与慢性牙周炎的关系以及在慢性牙周炎的诊断、预防、治疗中的最新进展做一综述。方法:由第一作者应用计算机在PubMed、Web of Science和CNKI等数据库检索涉及细胞外囊泡在慢性牙周炎防治中的相关研究,检索关键词为“extracellular vesicle,exosome,periodontal disease,immunomodulatory,outermembrane vesicles,bone regeneration”和“细胞外囊泡,外泌体,牙周病,免疫调节,外膜囊泡,骨再生”,检索时间范围为2014年4月至2021年10月。结果与结论:细胞外囊泡是几乎所有细胞都能产生的球囊状小体,其广泛存在于各种体液中,表面含有丰富的蛋白质、脂质以及遗传物质,通过介导细胞间的信息传递来影响靶细胞的生物学行为。一方面,慢性牙周炎的炎症反应离不开细菌分泌的细胞外囊泡的介导,其能够作为生物标志物来诊断、评估慢性牙周炎;另一方面,利用细胞外囊泡制备的新型慢性牙周炎疫苗初有成效,载有信息分子的细胞外囊泡还能够参与调节免疫反应,促进牙周组织的修复与再生。因此,在慢性牙周炎的组织损伤修复与再生领域,细胞外囊泡是一种极具潜力的无细胞新疗法,但其提取、纯化以及安全性仍然是临床转化前需要克服的问题。
BACKGROUND:Extracellular vesicles can control the inflammatory response by participating in immunomodulation of intercellular signaling,while also repairing periodontal tissue and promoting periodontal tissue regeneration.The application of this technology to the preventive diagnosis and tissue regeneration of chronic periodontitis is expected to become a method of cell-free tissue regeneration.OBJECTIVE:To review the synthesis and secretion of extracellular vesicles,biological function,relationship with chronic periodontitis,and recent advances in the diagnosis,prevention and treatment of chronic periodontitis.METHODS:The first author applied the computer to search for relevant studies involving extracellular vesicles in the prevention and treatment of chronic periodontitis in databases such as PubMed,Web of Science and CNKI.The search keywords were“extracellular vesicle,exosome,periodontal disease,immunomodulatory,outermembrane vesicles,bone regeneration”in Chinese and English.The retrieval time was from April 2014 to October 2021.RESULTS AND CONCLUSION:Extracellular vesicles are balloon-like bodies that can be produced by almost all cells,which are widely present in various body fluids,and are rich in proteins,lipids and genetic material on the surface,which affect the biological behavior of target cells by mediating information transfer between cells.On the one hand,the inflammatory response of chronic periodontitis is inseparable from the mediation of extracellular vesicles secreted by bacteria,which can be used as a biomarker to diagnose and evaluate chronic periodontitis;on the other hand,the new chronic periodontitis vaccine made by extracellular vesicles has initially been effective,and the extracellular vesicles containing information molecules can also participate in regulating the immune response and promoting the repair and regeneration of periodontal tissue.Therefore,in the field of tissue damage repair and regeneration of chronic periodontitis,extracellular vesicles are a new and potential cell
作者
刘卓冉
姜明
李友瑞
Liu Zhuoran;Jiang Ming;Li Yourui(Department of Prosthodontics,Affiliated Hospital of Binzhou Medical College,Binzhou 256600,Shandong Province,China;First Department of Dentistry and Endodontics,Jinan Stomatological Hospital,Jinan 250000,Shandong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第1期99-104,共6页
Chinese Journal of Tissue Engineering Research
关键词
细胞外囊泡
外泌体
牙周病
免疫调节
外膜囊泡
骨再生
综述
extracellular vesicle
exosome
periodontal disease
immunomodulation
outer membrane vesicle
bone regeneration
review
