摘要
目的评价不同保存条件下血脂检测结果的一致性。方法140份血样分离血清,样品分成5份,分别在即刻0h,4℃冷藏4h和24h,-20℃和-80℃冷冻保存30d检测血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)。采用Bland-Altman法和Passing-Bablock回归分析一致性。结果即刻0h检测分别与4℃4h、4℃24h,-20℃30d和.-80℃30d检测比较:TC、LDL-C数据使用差值Bland-Altman法分析,TC差值均值分别为0.00、0.00、0.07、0.03 mmol/L,LDL-C差值均值分别为-0.02、-0.04、-0.02、-0.07 mmol/L,95%的点在临床可接受一致性界值内。TG、HDL-C数据使用比值Bland-Altman法分析,TG比值均值分别为1.01、1.00、1.05、1.05,HDL-C比值均值分别为1.01.1.03.1.05.1.03,95%的点在临床可接受一致性界值内。结合2021年国家卫生健康委临床检验中心室间质量评价标准,血样不同保存条件下检测结果与即刻检测结果比对的Bland-Altman法分析一致性良好。结论大型人群流行病学调查中现场分离血清在4℃冷藏4h、24h,-20℃和-80℃冷冻保存30d后进行TC、LDL-C、HDL-C、TG检测均可得到与即刻检测一致的结果。
OBJECTIVE To evaluate the consistency of blood lipid test result under different storage conditions.METHODS Blood samples from 140 subjects were collected, centrifuged and were divided into five aliquots. One aliquot was used to determine serum total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C) immediately, and the other four parts were stored at 4 ℃ for 4 h, 24 h, and frozen at-20 ℃ and-80 ℃ for 30 d, respectively, for the further measurement.RESULTS Immediate 0 h detection was compared with 4 ℃ for 4 h and 24 h,-20 ℃and-80 ℃ for 30 d: The difference Bland-Altman method was used to analyze TC and LDL-C data, and the mean TC difference values was 0.00, 0.00,0.07 and 0.03 mmol/L, respectively. LDL-C difference value was-0.02,-0.04,-0.02 and-0.07 mmol/L, respectively, and 95% of the points were within the clinically acceptable conformance threshold. Ratio Bland-Altman method was used to analyze TG and HDL-C data. The mean TG ratios was 1.01, 1.00, 1.05, 1.05, and the mean HDL-C was 1.01, 1.03, 1.05 and 1.03, respectively. Ninety-five percent of the points were within the clinically acceptable consistency boundary. According to the 2021 Interlaboratory Quality Evaluation Standard of Clinical Laboratory Center of National Health Commission, the Bland-Altman analysis showed good consistency between the test result of different blood samples under different storage conditions and the immediate test result.CONCLUSION In large population epidemiological studies, the results of TC,LDL-C,HDL-C and TG in sera being refrigerated at 4 ℃ for 4 h and 24 h, and frozen at-20 ℃ and-80 ℃ for 30 d, were consistent with the result of immediate detection.
作者
满青青
李红
赵耀
李裕倩
刘贝贝
张坚
Man Qingqing;Li Hong;Zhao Yao;Li Yuqian;Liu Beibei;Zhang Jian(National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Beijing Center for Disease Control and Prevention,Beijing 100013,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2022年第3期490-496,共7页
Journal of Hygiene Research
基金
国家财政项目:公共卫生应急反应机制的运行(No.131031107000210002)。
