摘要
马铃薯作为西藏人民主要的粮食作物之一,在西藏具有重要的生产地位。本研究以脱毒组培苗“艾玛一号”和“200902”马铃薯为试验材料,比较并分析生长在MS和马铃薯2种不同培养基中叶片数、生根数和株高等性状,研究发现,生长于MS培养基中的马铃薯长势及所测指标均优于马铃薯培养基。再以MS为基础培养基,分别加入NAA、6-BA、2,4-D、玉米素和激动素,并各设置3种浓度梯度,一个月后对组培苗的性状进行统计研究,结果表明,植物生长调节剂在NAA 0.2 mg/L和NAA 0.1 mg/L的浓度时,更适合于“艾玛一号”和“200902”马铃薯生长,而低浓度的NAA对2种组培苗均有较好的扩繁效果。通过不同培养基及生长调节剂对马铃薯组培苗生长情况的研究,为脱毒马铃薯规模化生产提供理论依据。
As the one of the main food crops of the Tibetan people,potato has an important production status in Tibet.In this study,the potato virusfree tissue culture seedlings‘Ai Ma 1’and‘200902’were used as test materials.Firstly,the trait of leaf number,rooting number and plant height was compared between in MS and potato two different media.The study found that the growth vigor and the measured indicators of potato grown in MS medium were better than those of potato medium.Secondly,MS was used as the basal medium,and NAA,6-BA,2,4-D,Trans-Zeatin and kinetin were added respectively,and three concentration gradients were set for each.After one month,the characteristics of tissue culture seedlings were statistically studied.The results showed that the plant hormones at the concentrations of NAA 0.2 mg/L and NAA 0.1 mg/L were more suitable for the growth of‘Ai Ma 1’and‘200902’,respectively.While low concentrations of NAA were more suitable for expanding propagation of both tissue culture seedlings.Through the research on the growth of potato tissue culture seedlings with different media and hormones,it is beneficial to provide a theoretical basis for the large-scale production of virus-free potatoes.
作者
黄芩
任盼盼
何燕
HUANG Qin;REN Panpan;HE Yan(College of Tibet Agricultural and Animal Husbandry,Tibet Linzhi 860000,China)
出处
《西藏农业科技》
2022年第1期29-33,共5页
Tibet Journal of Agricultural Sciences
