摘要
开发一种pH值响应性羧甲基琼脂糖-聚多巴胺(carboxymethyl agarose-polydopamine,CMA-PDA)水凝胶载体。通过氯乙酸取代法合成了CMA。采用红外光谱、核磁共振、电子显微镜和热重分析对CMA的理化性质进行表征。进一步通过冷凝方法制备CMA-PDA水凝胶,系统研究CMA-PDA水凝胶的流变性能和质构性能进行表征。结果表明,PDA的加入提高了水凝胶的凝胶强度、硬度和黏弹性。以阿霉素为模型,研究该水凝胶在pH 2.0、6.2、6.8、7.4条件下的释放行为,表明其具有较好的pH值响应性,pH 2.0条件下的释放率显著高于其他条件。同时以L929细胞为模型,研究该水凝胶的生物相容性,表明其无明显细胞毒性。这项研究证明了CMA-PDA水凝胶生物相容性良好,具有pH值响应性和缓释性能,可以作为潜在的生物活性物质递送载体。
In this study,a pH responsive carboxymethyl agarose-polydopamine(CMA-PDA)hydrogel carrier was developed.Carboxymethyl agarose(CMA)was synthesized by replacing the hydroxyl groups of agarose with chloroacetic acid and was characterized by Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR),scanning electron microscopy(SEM),transmission electron microscopy(TEM)and thermal gravimetric analysis(TGA).Then,the CMA-PDA hydrogel was prepared,and its rheological and texture properties were characterized.The results showed that the gel strength,hardness and viscoelasticity of the hydrogel were increased by the addition of PDA.Doxorubicin(DOX)was used as a model to study the release behavior of the hydrogel at pH 2.0,6.2,6.8 and 7.4.The results showed that the hydrogel had good pH responsiveness and the release rate was significantly higher at pH 2.0 than at other pH levels.Moreover,the hydrogel had no cytotoxicity on L929 cells.This study proves that CMA-PDA hydrogel has good biocompatibility,pH responsive and slow release properties,and can be used as a potential carrier for bioactive substance delivery.
作者
郭雨宁
黄文灿
毛相朝
GUO Yuning;HUANG Wencan;MAO Xiangzhao(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第10期59-65,共7页
Food Science
基金
山东省泰山学者青年专家计划项目(tsqn201812020)。
