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慢性阻塞性肺疾病患者血浆中性粒细胞胞外诱捕网和白细胞介素-8及白细胞介素-33的表达水平及其临床意义 被引量:10

The expression and clinical significance of neutrophil extracellular traps,interleukin 8 and interleukin 33 in plasma of patients with chronic obstructive pulmonary disease
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摘要 目的探讨慢性阻塞性肺疾病(简称慢阻肺)患者血浆中中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)的主要成分双链DNA(double-stranded DNA,dsDNA)、瓜氨酸组蛋白3(citrulline histone 3,CitH3)、髓过氧化物酶DNA(myeloperoxidase-DNA,MPO-DNA)和白细胞介素(interleukin,IL)-8、IL-33的表达水平及其临床意义。方法选取2020年10月至2021年5月在石河子大学医学院第一附属医院住院的40例慢阻肺患者为急性加重期组,其经过治疗进入稳定期后纳入稳定期组,同期40例健康体检者为对照组。采集各组一般资料及外周血,通过酶联免疫吸附法测定血浆中CitH3、MPO-DNA、IL-8、IL-33水平,采用Pico Green荧光染料定量分析血浆中ds DNA水平,行肺功能检测。结果急性加重期组的ds DNA、Cit H3、MPO-DNA、IL-8、IL-33水平均高于稳定期组和对照组,差异均具有统计学意义(P<0.05);稳定期组dsDNA、CitH3、MPO-DNA、IL-33的水平均高于对照组,差异均具有统计学意义(P<0.05)。急性加重期组CitH3、MPO-DNA、IL-33和IL-8水平均与慢阻肺评估测试评分呈正相关。稳定期组MPO-DNA和IL-8水平均与慢阻肺评估测试评分呈正相关;稳定期组MPO-DNA与第1秒用力呼气容积占预计值百分比呈负相关;稳定期组CitH3与第1秒用力呼气容积与用力肺活量比值呈负相关。急性加重期组IL-8和dsDNA、CitH3水平呈正相关,与MPO-DNA水平无明显相关性;稳定期组IL-8和dsDNA水平呈正相关,与CitH3和MPO-DNA无明显相关性。急性加重期组IL-33和IL-8、dsDNA、CitH3、MPO-DNA水平呈正相关;稳定期组IL-33和IL-8、dsDNA、CitH3、MPO-DNA水平无明显相关性。结论慢阻肺患者血浆中NETs、IL-8、IL-33水平升高,且NETs水平与肺功能、慢阻肺评估测试评分、IL-33、IL-8有相关性,NETs可能参与了慢阻肺的发生发展。 Objective To investigate the expression of double-stranded DNA(dsDNA),citrulline histone 3(CitH3),myeloperoxidase-DNA(MPO-DNA),IL-8 and IL-33 in plasma of patients with chronic obstructive pulmonary disease(COPD)and their clinical significance.Methods Forty patients with acute exacerbation COPD(AECOPD)who were hospitalized in The First Affiliated Hospital of Shihezi University School of Medicine from October 2020 to May2021 were recruited in the AECOPD group,and recruited in the stable COPD group when they entered the stable stage.In the same period,forty healthy individuals were recruited in the control group.General informations including pulmonary function and peripheral blood were collected from each subject.Plasma levels of CitH3,MPO-DNA,interleukin(IL)-8and IL-33 were measured by enzyme linked immunosorbent assay and plasma levels of dsDNA were measured by PicoGreen fluorescent dye quantitative analysis.Results The levels of dsDNA,CitH3,MPO-DNA,IL-8 and IL-33 in the AECOPD group were higher than those in the stable group and the control group,with statistical significance(P<0.05),and the levels of dsDNA,CitH3,MPO-DNA and IL-33 in the stable group were higher than those in the control group,with statistical significance(P<0.05).The levels of CitH3,MPO-DNA,IL-33 and IL-8 in the AECOPD group were positively correlated with COPD assessment test(CAT)score.MPO-DNA and IL-8 were positively correlated with CAT score,MPO-DNA was negatively correlated with FEV1%pred,CitH3 was negatively correlated with FEV1/FVC.The levels of IL-8 and dsDNA,CitH3 were positively correlated with the levels of MPO-DNA in the AECOPD group,and positively correlated with the levels of IL-8 and dsDNA in the stable group,but not with CitH3 and MPO-DNA.The levels of IL-33and IL-8,dsDNA,CitH3,MPO-DNA were positively correlated in the AECOPD group,but not in the stable group.Conclusions The levels of neutrophil extracellular traps(NETs),IL-8 and IL-33 in plasma of COPD patients were increased,and the levels of NETs were correlated with pulmonar
作者 范傲 黄钟 段宇清 卢献灵 FAN Ao;HUANG Zhong;DUAN Yuqing;LU Xianling(First Department of Respiratory Medicine,The First Affiliated Hospital of Shihezi Medical University,Shihezi,Xinjiang 832000,P.R.China;Emergency Department,The First Affiliated Hospital of Shihezi Medical University,Shihezi,Xinjiang 832000,P.R.China;NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases,First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,Xinjiang 832000,P.R.China)
出处 《中国呼吸与危重监护杂志》 CAS CSCD 北大核心 2022年第2期84-89,共6页 Chinese Journal of Respiratory and Critical Care Medicine
基金 石河子大学科研项目(ZZZC202078 B) 中国医学科学院中央级公益性科研院所基本科研业务费专项资金资助(2020-PT330-003)。
关键词 慢性阻塞性肺疾病 中性粒细胞胞外诱捕网 白细胞介素-8 白细胞介素-33 Chronic obstructive pulmonary disease neutrophil extracellular traps interleukin-8 interleukin-33
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