摘要
目的运用网络药理学方法揭示葛根芩连汤治疗放射性肠炎的作用机制。方法运用中药系统药理学数据库与分析平台(TCMSP)获取葛根芩连汤有效成分及活性成分作用靶点;运用GeneCards数据库获取放射性肠炎疾病基因;将靶点和靶标基因取交集,获得关键靶点,用String数据库对关键靶点构建蛋白质-蛋白质相互作用网络。运用DAVID 6.8数据库对关键靶点进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)通路富集分析。结果共收集葛根芩连汤138个活性成分和8个关键作用靶点。GO富集分析和KEGG通路富集分析其主要机制是通过调控黏附斑激酶(FAK)及其信号通路,MAPK信号通路等信号通路,调节Th17细胞分化在放射性肠炎损伤修复中发挥损伤修复作用。结论葛根芩连汤治疗放射性肠炎可能影响了放射性肠炎的免疫微环境,并调控肠道炎症反应起到治疗作用。
Objective To explain why Gegen Qinlian Decoction(GQD)had effects on the treatment of radiation proctitis(RP)in a comprehensive and systematic way by network pharmacology.Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)were used to collect the compound-targets networks.The Gene Cards was used to obtained the relevant target genes of RP.The compound-targets and RP related target genes were taken to obtain the key role of the treatment of RP.The String database was used to construct a protein interaction network for the key role.Gen ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment were analyzed on key targets by DAVID and ClueGo.Results A total of 138 active ingredients and 8 key targets of GQD were collected.The main mechanism throuth GO analysis and KEGG pathway enrichment analysis were the regulation of focal adhesion kinase(FAK)and its signaling pathway,MAPK signaling pathway and regulation of Th17 cell differentiation,which play roles in radiation damage repair of RP.Conclusion The possible mechanism of GQD in the treatment of RP mainly includes infection of the immune microenvironment,and the inflammatory response of intestinal tract.
作者
朱旭
张博
张思萌
夏晓萌
吴登斌
ZHU Xu;ZHANG Bo;ZHANG Si-Meng;XIA Xiao-Meng;WU Deng-Bin(Department of radiation oncology General Hospital of Angang Group,Anshan 114000,China;Graduate School Liaoning University of traditional Chinese medicine,Shenyang 110000,China)
出处
《中国药物经济学》
2022年第3期27-30,共4页
China Journal of Pharmaceutical Economics
基金
辽宁省自然科学基金指导计划(20180550798)。
关键词
葛根芩连汤
放射性肠炎
网络药理学
Gegen Qinlian Decoction
Radiation Proctitis
Network Pharmacology
