摘要
为探索正茬与迎茬种植方式对大豆根际土壤微生物群落组成的影响,分析导致大豆迎茬障碍形成的主要病原微生物,研究大豆迎茬障碍的形成机制,进而为其生物防治提供一定的理论基础,本研究采用Illumina HiSeq 2500高通量测序技术分析正茬与迎茬大豆根际土壤微生物群落组成。结果表明:大豆迎茬种植在一定程度上降低了土壤细菌群落丰度和多样性,而增加了土壤真菌群落丰度和多样性。正茬与迎茬大豆根际土壤微生物群落组成和主要优势菌群所占比例均存在较大差异。酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria)分别为正茬和迎茬大豆根际土壤细菌群落中最具优势的细菌门。镰孢霉属(Fusarium)和粗糙孔菌属(Subulicystidium)分别为正茬和迎茬大豆根际土壤真菌群落中最具优势的属。
In order to explore the effects of planting methods on the composition of microbial communities in the rhizosphere soil of soybean,we analyzed the main pathogenic microorganisms leading to the formation of soybean alternate cropping obstacle,and explore the formation mechanism of soybean alternate cropping obstacle,so as to provide a theoretical basis for its biological control.We used Illumina HiSeq 2500 high-throughput sequencing technology to analyze the effects of rotational cropping and alternate cropping on the composition of microbial communities in the rhizosphere soil of soybean.The results showed that the abundance and diversity of bacterial communities in the rhizosphere soil of alternate cropping soybean were decreased,but the abundance and diversity of fungal communities were increased.There were significant differences in the composition of microbial communities and the proportion of dominant microflora in the rhizosphere soil of rotational cropping and alternate cropping soybean.Acidobacteria and actinobacteria were the most dominant bacterial phylum in the rhizosphere soil of rotational cropping and alternate cropping soybean,respectively.Fusarium and Subulicystidium were the most dominant fungal genera in the rhizosphere soil of rotational cropping and alternate cropping soybean,respectively.
作者
接伟光
杨冬莹
姚延轩
林厚泽
JIE Wei-guang;YANG Dong-ying;YAO Yan-xuan;LIN Hou-ze(School of Food Engineering,Heilongjiang East University,Harbin 150066,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2022年第2期179-188,共10页
Soybean Science
基金
黑龙江省自然科学基金优秀青年项目(YQ2019C016)。
关键词
正茬
迎茬
大豆
根际土壤
微生物群落组成
高通量测序
rotational cropping
alternate cropping
soybean
rhizosphere soil
composition of microbial communities
high-throughput sequencing
