摘要
目的采用超高效液相色谱-离子阱-静电场轨道阱质谱(UPLC-LTQ-Orbitrap-MS)技术分析鉴定加味参芪地黄汤中化学成分。方法采用Warters ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)色谱柱,流动相:0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B),梯度洗脱,流速为0.3 ml/min。质谱采用电喷雾(ESI)离子源及静电场轨道离子阱质量分析器,采集质谱碎片信息为正、负离子模式,通过与对照品的相对保留时间进行比对,并根据质谱碎片信息进行分析,同时,结合文献报道,实现对加味参芪地黄汤中化学成分的准确鉴定。结果研究发现加味参芪地黄汤水煎剂采用UPLC-LTQOrbitrap-MS技术共鉴定出62个化学成分,包括13个芳香酸类、9个黄酮类、8个皂苷类成分、5个芳香胺类、3个酮酸类、2个酚类、1个芳香醌类及其他成分。结论本研究所采用的鉴定分析方法高效、准确,可应用于加味参芪地黄汤中化学成分的鉴定分析,为其后期的药效物质基础作用机制提供重要的实验数据。
Objective To analyze the chemical compounds of Shenqi Dihuang decoction by the ultraperformance liquid chromatography coupled with linear quadrupole ion trap-orbitrap mass spectrometry(UPLC-LTQ-Orbitrap-MS).Methods Warters ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)was used as chromatographic column with mobile phase:0.1%formic acid water(A)-0.1%formic acid acetonitrile(B)with gradient elution,and flow rate was 0.3 ml/min.Electrospray ion source(ESI)and an electrostatic field orbital ion trap mass analyzer were adopted,which was used to collect mass spectrometry fragment information with positive and negative ion modes,by comparing with the relative retention time of the reference substance.In addition,the fragment information of the mass spectrum was used to identify the compounds.The accurate identification of the chemical components in Shenqi Dihuang decoction was confirmed with literature.Results The study found that UPLC-LTQ-Orbitrap-MS technology could be used to identify 62 chemical components,including 13 aromatic acids,9 flavonoids,8 saponins,and 5 aromatic amines,3 keto acids,2 phenols,1 aromatic quinone and other ingredients in Shenqi Dihuang decoction.Conclusion The identification analysis method in this study was efficient and accurate,which could be applied to the identification and analysis of chemical components in Shenqi Dihuang decoction and provided the important experimental data for the research on the material basis and mechanism.
作者
万众
刘王振祖
谈鸣岳
徐东亮
胡诚
纪万里
WAN Zhong;LIU Wangzhenzu;TAN Mingyue;XU Dongliang;HU Cheng;JI Wanli(Urinary Center,Shuguang HospitalAffiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai University of Traditional Chinese Medicine School of PharmacySchool of Pharmacy,Shanghai 201203,China;Shanghai University of Traditional Chinese Medicine School of Pharmacy Science and Technology Experimental Center,Shanghai 201203,China;School of Pharmacy,Shanghai University of Medicine&Health Sciences,Shanghai 201318,China)
出处
《药学实践杂志》
CAS
2022年第3期231-237,264,共8页
Journal of Pharmaceutical Practice
基金
国家自然科学基金项目(82174122)。
关键词
加味参芪地黄汤
化学成分
超高效液相色谱-离子阱-静电场轨道阱质谱
Shenqi Dihuang decoction
chemical constituents
ultra performance liquid chromatography-ion trap-electrostatic field orbitrap mass spectrometry
