摘要
目的 探讨肥胖相关蛋白(FTO)在前列腺癌组织和细胞中的表达及对前列腺癌细胞侵袭功能的影响。方法 采用免疫组织化学染色方法检测前列腺癌组织中FTO的表达;采用蛋白免疫印迹法(Western blot)和实时荧光定量PCR(qPCR)检测FTO在前列腺正常细胞和癌细胞中的表达;分析FTO的表达与前列腺癌组织的临床病理特征的关系;采用FTO慢病毒干扰载体(pLVX-shRNA-FTO)感染前列腺癌细胞22RV1后,Western blot检测实验组、对照组和空白组的FTO蛋白表达水平,Transwell实验检测细胞的侵袭能力。结果 前列腺癌组织中FTO蛋白表达水平明显高于癌旁组织(P<0.05);前列腺癌细胞中FTO的蛋白和mRNA表达水平高于前列腺正常细胞(P<0.05);FTO的表达与前列腺癌组织的Grade分级有关(P<0.05);实验组FTO蛋白表达水平低于对照组和空白组(P<0.05);下调FTO的表达能够抑制前列腺癌细胞22RV1的侵袭能力。结论 FTO在前列腺癌组织和细胞中高表达,其能促进前列腺癌细胞的侵袭能力,FTO可能是前列腺癌发生发展的关键分子。
Objective To investigate the expression of obesity-related protein(FTO)in prostate cancer tissues and cells and its effect on the invasion function of prostate cancer cells.Methods Immunohistochemical staining was used to detect the expression of FTO in prostate cancer tissue.Western blot and real-time PCR(qPCR)were used to detect the expression of FTO in prostate normal cells and cancer cells.The relationship between the expression of FTO and the clinicopathological characteristics of prostate cancer tissue was analyzed.After the prostate cancer cell 22RV1 was infected with FTO lentiviral vector(pLVX-shRNA-FTO),the expression levels of FTO protein in the experimental group,the control group and the blank group were detected by Western blot,and the invasive ability of the cells was detected by Transwell assay.Results The protein expression level of FTO in prostate cancer tissues was significantly higher than that in adjacent tissues(P<0.05);the protein and mRNA expression levels of FTO in prostate cancer cells were higher than those in normal prostate cells(P<0.05);the expression of FTO was related to the grade of prostate cancer tissue(P<0.05);the expression level of FTO protein in the experimental group was lower than that in the control group and blank group(P<0.05);down-regulation of FTO expression significantly inhibited the invasive ability of prostate cancer cells 22RV1.Conclusion FTO is highly expressed in prostate cancer tissues and cells,and it can promote the invasion ability of prostate cancer cells.FTO may be a key molecule in the occurrence and development of prostate cancer.
作者
胡萍
李会
杨溪霖
张海宁
卿克勤
HU Ping;LI Hui;YANG Xilin;ZHANG Haining;QING Keqin(Department of Clinical Laboratory,Chengdu First People's Hospital,Chengdu,Sichuan 610041,China)
出处
《国际检验医学杂志》
CAS
2022年第10期1202-1206,共5页
International Journal of Laboratory Medicine
基金
四川省成都市医学科研课题(2018062)。
关键词
前列腺癌
肥胖相关蛋白
侵袭
prostate cancer
obesity-related proteins
invasion
