摘要
从野菊(Chrysanthemum indicum)中克隆了cin-miR396a前体序列及其启动子,对前体序列进行比对与进化分析,对启动子顺式作用元件进行分析,并研究了cin-miR396a前体序列对拟南芥的遗传转化及对其耐盐性的影响。结果表明,cin-MIR396a长度为145 bp,含有完整的茎环结构,与拟南芥ath-MIR396a进化关系最近。启动子区具有光信号、茉莉酸甲酯、赤霉素、水杨酸、脱落酸等多种响应元件,推测cin-MIR396a参与多种逆境胁迫下的应答及调控。在拟南芥中过表达cin-miR396a前体可以提高盐胁迫下种子发芽率以及成苗期的耐盐性,cin-miR396a在拟南芥响应盐胁迫中发挥正调控作用。
cin-miR396a precursor sequence and its promoter were cloned from Chrysanthemum indicum;the precursor sequence alignment and evolutionary analysis were performed;the promoter was analyzed for cis-acting elements.The genetic transformation of the cin-miR396a precursor sequence to Arabidopsis thaliana was further carried out,and the salt tolerance of transgenic Arabidopsis thaliana was analyzed.The results showed that cin-MIR396a was 145 bp in length,contained an intact stem-loop structure,and was evolutionarily closest to A.thaliana ath-MIR396a.The promoter region has multiple response elements such as light signal,methyl jasmonate,gibberellin,salicylic acid,and abscisic acid,and it is hypothesized that cin-MIR396a is involved in response and regulation under a variety of adversity stresses.Overexpression of the cin-miR396a precursor in A.thaliana can improve seed germination under salt stress and salt tolerance at the adult seedling stage,and cin-miR396a plays a positive regulatory role in A.thaliana in response to salt stress.
作者
刘筱玮
夏斌
李子葳
杨宇佳
陈斌
周蕴薇
何淼
LIU Xiaowei;XIA Bin;LI Ziwei;YANG Yujia;CHEN Bin;ZHOU Yunwei;HE Miao(College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China;College of Horticulture,Jilin Agricultural University,Changchun 130118,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第4期816-826,共11页
Acta Horticulturae Sinica
基金
国家重点研发计划项目(2018YFD1000406,2019YFD1001504)
国家自然科学基金项目(31870687)。
关键词
野菊
miR396
拟南芥
转基因
盐胁迫
Chrysanthemum indicum
miR396
Arabidopsis thaliana
transgenic
salt stress
