摘要
为探究同一碳氮比条件下,不同碳源有机物料对植烟土壤碳氮、细菌群落及烟草生长发育的影响,采用盆栽试验,分别添加生物炭、腐熟小麦秸秆、腐熟牛粪、沼渣4种有机物料,将其与无机氮(化肥)混合调至碳氮比为30,研究其对植烟土壤碳氮及细菌群落的影响。结果表明,有机物料的施用可降低土壤容重、硝态氮含量,增加碱解氮、铵态氮含量,增加土壤总碳、总氮含量。腐熟小麦秸秆、腐熟牛粪的施用增加土壤微生物量碳氮含量,可为土壤微生物的生长发育提供良好的环境条件,进而影响土壤细菌多样性及群落结构。就不同碳源有机物料添加的整体效果来看,以腐熟小麦秸秆与无机氮配施对植烟土壤理化性质及微生物多样性的改善效果最好。该处理与对照(单施氮肥)相比,烟草移栽后60 d时,土壤容重降低了9.22%,总碳、总氮、微生物量碳、微生物量氮、细菌OTU(操作分类单元)数分别增加了44.39%、21.06%、49.22%、53.37%、6.70%;土壤细菌优势菌群为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、酸杆菌门(Acidobacteriota)、芽单胞菌门(Gemmatimonadota)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、黏球菌门(Myxococcota)。
In order to explore the effects of organic materials with different carbon sources on carbon and nitrogen,bacterial communities and tobacco growth and development in tobacco-planting soil under the same carbon-nitrogen ratio,a pot experiment was conducted,adding biochar,decomposed wheat straw,decomposed cow dung and biogas residue,respectively.Four kinds of organic materials were mixed with inorganic nitrogen(nitrogen fertilizer)and adjusted to the carbon-nitrogen ratio of 30,and their effects on carbon and nitrogen and bacterial communities in tobacco-growing soil were studied.The results showed that the application of organic materials reduced soil bulk density and nitrate nitrogen content,increased the content of alkaline hydrolyzed nitrogen and ammonium nitrogen,and increased soil total carbon content and total nitrogen content.The application of decomposed wheat straw and decomposed cow dung increased soil microbial biomass carbon and nitrogen.It provided good environmental conditions for the growth and development of soil microorganisms,which in turn affected soil bacterial diversity and community structure.In terms of the overall effect of adding organic materials with different carbon sources,the combined application of decomposed wheat straw and inorganic nitrogen had better effects on improving the physicochemical properties and microbial diversity of tobacco-growing soil.Compared with the control(single application of nitrogen fertilizer),the soil bulk density decreased by 9.22%at 60 days after tobacco transplanting,and the total carbon,total nitrogen,microbial biomass carbon,microbial biomass nitrogen and bacterial OTUs(Operational taxonomic units)increased by 44.39%,21.06%,49.22%,53.37%,6.70%respectively;The dominant flora of soil bacteria were Actinobacteriota,Proteobacteria,Chloroflexi,Acidobacteriota,Gemmatimonadota,Firmicutes,Bacteroidota and Myxococcota.
作者
张蕤
王欢欢
赵园园
程玉渊
吴疆
史宏志
ZHANG Rui;WANG Huanhuan;ZHAO Yuanyuan;CHENG Yuyuan;WU Jiang;SHI Hongzhi(Tobacco College of Henan Agricultural University/National Tobacco Cultivation and Physiology and Biochemistry Research Center,Zhengzhou 450002,China;Nanyang City Company of Henan Tobacco Company,Nanyang 473000,China)
出处
《河南农业科学》
北大核心
2022年第3期84-94,共11页
Journal of Henan Agricultural Sciences
基金
中国烟草总公司科技重点项目(110201902004)
河南省烟草公司南阳市公司科技项目(2020411300270065)
河南中烟工业有限责任公司科技项目(AW202142)。
关键词
烟草
有机物料
土壤碳氮
土壤微生物量碳氮
土壤细菌群落
Flue-cured tobacco
Organic materials
Soil carbon and nitrogen
Soil microbial biomass carbon and nitrogen
Soil bacterial community
