摘要
通过对43份枸杞材料进行表型性状指标的测定,结合SSR分子标记技术,综合分析枸杞古树与现有宁夏枸杞品种(系)之间的群体结构和遗传多样性。表型性状主成分分析结果确定了主成分为4,累计贡献率达到78.09%,第一主成分叶片大小和第二主成分果实大小起主要贡献作用。样品变异系数范围为15%~54%,平均值为26.78%,样品遗传变异指数范围为5.26~5.41。表型性状指标通过聚类分析后分为4个类群,第Ⅰ类群3份材料,第Ⅱ类群3份材料,第Ⅲ类群8份材料,第Ⅳ类群29份材料。采用隶属函数法分析确定综合性状优良的5个材料,分别为L14、L16、L28、L40、L43。SSR分子标记遗传信息有效等位基因平均值为2.018,期望杂合度平均值为0.398,Shannon′s指数平均值为0.839。SSR分子标记确定最优K值为4,综合评价较好的5份材料中,L16表现为杂合基因型,L14、L28、L40、L43表现为单一基因型。本研究运用多种方法综合分析了不同来源、不同树龄枸杞古树的表型性状差异和遗传多样性,为后续枸杞古树种质资源的利用及枸杞品种选育提供了理论依据和实践指导。
Through the determination of phenotypic traits of 43 wolfberry materials,combined with SSR molecular marker technology,the population structure and genetic diversity between ancient wolfberry trees and existing wolfberry varieties in Ningxia were comprehensively analyzed. The principal component analysis results of phenotypic traits determined that the principal component was 4,and the cumulative contribution rate reached 78.09%. The leaf size of the first principal component and the fruit size of the second principal component played a major role. The coefficient of variation ranged from 15% to 54%,with an average of 26.78%,and the genetic variation index ranged from 5.26 to 5.41. The phenotypic traits were divided into 4 groups by cluster analysis,3 individuals in group Ⅰ,3 individuals in group Ⅱ,8 individuals in group Ⅲ and 29 individuals in group Ⅳ. Five materials with excellent comprehensive properties were determined by membership function method,namely L14,L16,L28,L40 and L43. The average effective alleles of SSR molecular markers were 2.018,the average expected heterozygosity was 0.398,and the average Shannon’s index was 0.839. The optimal K value of SSR molecular markers was 4. Among the five materials with better comprehensive evaluation,L16 showed heterozygous genotype,and L14,L28,L40 and L43 showed single genotype. In this study,a variety of methods were used to comprehensively analyze the phenotypic traits and genetic diversity of ancient wolfberry trees from different sources and ages,which provided a theoretical basis and practical guidance for the subsequent utilization of ancient wolfberry germplasm resources and the breeding of wolfberry varieties.
作者
胡永超
马洁
唐建宁
朱金忠
杨涓
郑蕊
张磊
郑国琦
HU Yong-chao;MA Jie;TANG Jian-ning;ZHU Jin-zhong;YANG Juan;ZHENG Rui;ZHANG Lei;ZHENG Guo-qi(College of Life Sciences,Ningxia University,Yinchuan 750021;Ningxia Forestry and Grassland Bureau Ningxia Lyciumbarbarum L.Industry Development Center,Yinchuan 750021;Ningxia Zhongning County Qixin Lycium barbarum L.Seed Industry Limited Company,Zhongning 755100;School of Biology and Engineering,North University for Nationalities,Yinchuan 750021)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第3期755-767,共13页
Journal of Plant Genetic Resources
基金
宁夏回族自治区自然科学基金(2021AAC02005,2022AAC05036,2020AAC03097)
国家自然科学基金(81760682)
宁夏枸杞产业发展中心项目(022004089013)。
关键词
枸杞古树
表型性状
SSR标记
遗传多样性
ancient trees with different ages of Lycium barbarum L
phenotypic character
SSR molecular marker
genetic diversity
