摘要
目的探究基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)分析耐碳青霉烯类肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKP)同源性在临床中的应用价值。方法收取某院2018年9月—2020年10月临床分离的肺炎克雷伯菌非重复菌株295株,经耐药表型筛选出CRKP;利用MALDI-TOF MS采集CRKP的质谱图,分别运用SARAMIS软件中的相似性分型和identical masses分型进行同源性分析;以脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)为参考标准,将CRKP分为高度、中度、低度同源性3组,评价MALDI-TOF MS在分析CRKP同源性分析中的应用价值。结果经耐药表型筛选出55株CRKP;3组同源性分析显示:高度同源组中,应用SARAMIS软件自带的identical mass分型和相似性分型分析,大多identical mass集中分布(60%~80%),相似性>80%,与PFGE结果较为一致;中度同源组中,identical mass>70%,相似性>85%,显示菌株间高度集中,跟PFGE结果存在较大的差异;低度同源组中,identical mass<70%,相似性<80%,与PFGE结果类似,能较好体现菌株间的差别。结论MALDITOF MS自带的SARAMIS软件目前仅在高度同源和低度同源菌株中,可作为分析同源性的初筛方式。
Objective To explore the clinical applicability of matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS)in the analysis of carbapenem-resistant Klebsiella pneumoniae(CRKP).Methods A total of 295 Klebsiella pneumoniae non repetitive strains were collected from a hospital from September 2018 to October 2020,and CRKP was screened by drug resistance phenotype;the mass spectra of CRKP were collected by MALDI-TOF MS,the similarity and identity masses in SARAMIS software were used to analyze the homology;using pulsed field gel electrophoresis(PFGE)as reference standard,CRKP were divided into three groups:High,medium and low degree of homology,and MALDI-TOF MS was applied in CRKP homology analysis.Results 55 CRKP strains were screened by drug resistance phenotype;three groups of homology analysis showed that:In the highly homologous group,most of them were distributed in a centralized manner(60%~80%)with similarity>80%,which was consistent with PFGE results;in the moderate homology group,the identity mass value was more than 70%,and the similarity was more than 85%.It showed that the strains were highly concentrated,which was different from the results of PFGE;in the low degree homology group,the identity mass was less than 70%,and the similarity was less than 80%,which was similar to that of PFGE.Conclusion At present,the SARAMIS software provided by MALDI-TOF MS is only used in high homologous and low homologous strains,which can be used as the primary screening method for analyzing the homology.
作者
张杰
张金鑫
楚新旭
应冲涛
郭普
汪华学
Zhang Jie;Zhang Jin-xin;Chu Xin-xu;Ying Chong-tao;Guo Pu;Wang Hua-xue(Intensive Care Unit,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004;Department of Pain,the First Affiliated Hospital of Bengbu Medical College Bengbu 233004;Department of Clinical Medical Laboratory,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004)
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2022年第4期381-387,共7页
Chinese Journal of Antibiotics
基金
安徽省重点研究与开发计划项目(No.1804h08020256)。
