摘要
针对冬春季节西北地区设施栽培辣椒生产过程中出现的低温弱光逆境问题,本研究以低温敏感型辣椒品种‘航椒2号’为试材,开展不同浓度外源玉米黄质对低温弱光胁迫下辣椒幼苗缓解效应的研究。辣椒幼苗通过连续4d叶面喷施0(CK)、20(T1)、50(T2)、100(T3)mg·L^(-1)的玉米黄质溶液预处理,后置于人工气候箱模拟低温弱光胁迫处理7 d,测定辣椒幼苗生长、光合和抗氧化酶相关生理指标。研究发现不同浓度外源玉米黄质预处理均对低温弱光条件下辣椒幼苗的胁迫有明显的缓解作用,其中以T2处理效果最佳。与CK相比,T2处理株高和茎粗分别增加5.52%和17.09%;叶片光合色素含量及净光合速率(P_(n))、气孔导度(G_(s))、蒸腾速率(T_(r))均显著提高;渗透调节物质可溶性糖和可溶性蛋白分别增加22.35%和19.58%;抗氧化酶SOD和CAT分别增加19.23%和381.55%。此外,T2处理显著降低了超氧阴离子、丙二醛(MDA)含量和相对电导率。表明50 mg·L^(-1)的外源玉米黄质能有效缓解低温弱光胁迫对辣椒幼苗造成的氧化损伤,保护细胞膜的完整性,减轻幼苗叶片光合器官的伤害,增强叶片气孔导度和光合能力,进而提高对低温弱光的耐受性。
To solve the problem of low temperature and weak light stress during the facility pepper production in northwest China in winter and spring,the study took the low temperature sensitive pepper variety‘Hangjiao No.2’as the test material to study the alleviating effect of exogenous zeaxanthin in different concentrations on pepper seedlings under low temperature and weak light stress.Pepper seedlings were pretreated with zeaxanthin solution of 0(CK),20(T1),50(T2)and 100(T3)mg·L^(-1) for 4 consecutive days,and then placed in an artificial climate chamber to simulate low temperature and weak light stress for 7 days.The physiological indexes related to growth,photosynthesis and antioxidant enzymes of pepper seedlings were measured.It was found that pretreatment with different concentrations of exogenous zeaxanthin significantly alleviated the stress of pepper seedlings under low temperature and light stress,and the T2 treatment had the best effect.Compared with CK,the plant height and stem diameter of the T2 treatment increased by 5.52%and 17.09%,respectively.The photosynthetic pigment content,net photosynthetic rate(P_(n)),stomatal conductance(G_(s))and transpiration rate(T_(r))of leaves were significantly increased.The soluble sugar and soluble protein of osmotic regulation substance increased by 22.35%and 19.58%,respectively.Antioxidant enzymes SOD and CAT increased by 19.23%and 381.55%,respectively.In addition,the T2 treatment significantly reduced the content of superoxide anion,malondialdehyde(MDA)and relative conductivity.The result showed that exogenous zeaxanthin(50 mg·L^(-1))effectively alleviated the oxidative damage caused by low temperature and weak light pepper seedlings,protected the integrity of the cell membrane,reduced the damage to photosynthetic organs of seedlings,strengthened the stomatal conductance and photosynthetic capacity of leaves,and improved the tolerance at low temperature and weak light stress.
作者
丁东霞
李静
颉建明
李能慧
韩康宁
张婧
牛天航
杨滟
DING Dongxia;LI Jing;XIE Jianming;LI Nenghui;HAN Kangning;ZHANG Jing;NIU Tianhang;YANG Yan(College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China)
出处
《干旱地区农业研究》
CSCD
北大核心
2022年第3期56-63,共8页
Agricultural Research in the Arid Areas
基金
甘肃省自然基金项目(20JR10RA515)
国家自然科学基金(32072657)
甘肃省瓜菜产业技术体系专项资金(GARS-GC-1)
国家重点研发计划(2016YFD0201005)
甘肃省教育厅优秀研究生“创新之星”项目(2021CXZX-409)。
关键词
玉米黄质
辣椒幼苗
低温弱光
光合参数
氧化胁迫
zeaxanthin
pepper seedling
low temperature and weak light
photosynthetic parameters
oxidative stress
