摘要
背景:有文献指出电刺激可以促进骨再生,具有生物电活性的压电材料可以应用于骨组织工程中。目的:研制一种能促进骨组织再生的压电薄膜材料,表征其对MC3T3-E1细胞黏附、增殖和成骨分化能力的影响。方法:以聚乳酸为原料,加入一定质量比例(0%,5%,10%,20%,30%,40%)的压电陶瓷钛酸钡,选择二氯甲烷为溶剂,通过溶液浇铸法制备钛酸钡/聚乳酸复合薄膜材料,电晕极化处理前后,表征其表面形貌、晶相构成、亲水性、压电性能,筛选出理化性能较优的材料。将极化处理前后的0%,20%钛酸钡/聚乳酸复合薄膜材料分别与MC3T3-E1细胞共培养,检测细胞的增殖、黏附与成骨分化能力。结果与结论:①扫描电镜、X射线衍射、水接触角实验及压电电常数检测结果显示,当加入20%的压电陶瓷钛酸钡时,极化处理的复合薄膜材料具有良好的理化性能,极化后压电常数d33达到(7.03±0.26)pC/N,处于骨组织正常压电范围;②CCK-8实验结果显示,培养第4天时,极化20%组细胞增殖活性高于未极化20%组(P<0.05);培养第7天时,极化20%组细胞增殖活性高于空白对照组、未极化0%组和未极化20%组(P<0.05);③荧光显微镜与扫描电镜下可见,极化20%组细胞呈多边形且伪足明显,较未极化20%组、极化0%组和未极化0%组体现出更好的细胞黏附能力;④碱性磷酸酶活性检测结果显示,培养第4天时,极化20%组的碱性磷酸酶活性高于未极化0%组、未极化20%组(P<0.05);培养第7天时,极化20%组的碱性磷酸酶活性高于未极化0%组、未极化20%组、极化0%组(P<0.05);⑤结果表明,钛酸钡/聚乳酸复合压电薄膜材料具有较好的压电性能和细胞相容性。
BACKGROUND:Electrical stimulation has been shown to promote bone regeneration and piezoelectric materials with bioelectrical activity can be used in bone tissue engineering.OBJECTIVE:To prepare an ideal piezoelectric film material,which can promote bone regeneration and characterize its effects on the adhesion,proliferation,and osteogenic differentiation of MC3T3-E1 cells.METHODS:Barium titanate/polylactic acid composite film was prepared by solution casting with polylactic acid as raw material and a certain proportion(0%,5%,10%,20%,30%,and 40%)of piezoelectric ceramic barium titanate was added and methylene chloride was selected as the organic solvent.After corona polarization treatment,the surface morphology,crystal phase composition,hydrophilic and piezoelectric properties of films were investigated to select materials with better physical and chemical properties.The 0%and 20%barium titanate/polylactic acid composite film materials before and after polarization treatment were separately co-cultured with MC3T3-E1 cells to test the ability of cell adhesion,proliferation,and osteogenic differentiation.RESULTS AND CONCLUSION:(1)The scanning electron microscope,X-ray diffraction,water contact angle experiment,and pressure point constant test results showed that when the mass ratio of barium titanate to composite film was 20%,the polarized composite film material had good physical and chemical properties.The piezoelectric constant d33 after polarization reached(7.03±0.26)pC/N,which was in the normal piezoelectric range of bone tissue.(2)CCK-8 assay results showed that on the 4th day of culture,the proliferation activity of the cells in the polarized 20%group was higher than that in the unpolarized 20%group(P<0.05).On the 7th day of culture,the proliferation activity of the cells in the polarized 20%group was higher than that of the blank control group,the unpolarized 0%group,and the unpolarized 20%group(P<0.05).(3)The fluorescence microscope and scanning electron microscope exhibited that the cells in the polarized
作者
代香林
张文凤
姚喜军
商佳琪
黄秋瑾
任怡帆
邓久鹏
Dai Xianglin;Zhang Wenfeng;Yao Xijun;Shang Jiaqi;Huang Qiujin;Ren Yifan;Deng Jiupeng(School of Stomatology,North China University of Science and Technology,Tangshan 063200,Hebei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第3期367-373,共7页
Chinese Journal of Tissue Engineering Research
基金
大中学生科技创新能力培育专项(2021H020909),项目负责人:张文凤。
关键词
引导性骨组织再生
压电材料
钛酸钡
聚乳酸
溶液浇铸
复合薄膜材料
电晕极化
压电性能
成骨分化
conductive bone tissue regeneration
piezoelectric material
barium titanate
polylactic acid
solution-casting
composite film material
corona polarization
piezoelectric properties
osteogenic differentiation
