摘要
从河南省济源市猪场仔猪粪便中筛选得到1株产β-甘露聚糖酶的菌株,通过外观形态学分析、生理生化结果分析、16S rDNA序列分析及构建系统发育树结果,鉴定该菌株为沙福芽孢杆菌(Bacillus safensis),命名为WBT0011。通过β-甘露聚糖酶和魔芋胶、瓜尔豆胶制备甘露寡糖,发现魔芋胶和瓜尔豆胶降解为多种甘露寡糖产物,说明二者水解较为完全,本研究旨在为β-甘露聚糖酶的应用及进一步工业化生产甘露寡糖的工艺优化提供帮助。
A strain producingβ-mannanase was screened from the feces of piglets in Jiyuan city,Henan province.The strain was identified as Bacillus safensis and named WBT0011 by morphological analysis,physiological and biochemical analysis,16S rDNA sequence analysis and phylogenetic tree construction.Mannan oligosaccharides were prepared byβ-mannanase,konjac glucomannan and guar gum.It was found that konjac glucomannan and guar gum were degraded into a variety of oligosaccharide products,indicating that they were hydrolyzed completely.It is hoped to provide help for the application ofβ-mannanase and the optimization of industrial production of mannan oligosaccharides.
作者
王佰涛
杨文玲
权淑静
雷高
李珊珊
刘德海
WANG Baitao;YANG Wenling;QUAN Shujing;LEI Gao;LI Shanshan;LIU Dehai(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou,Henan Province 450008,China)
出处
《中国饲料》
北大核心
2022年第9期47-51,56,共6页
China Feed
基金
中原科技创新领军人才计划资助项目(214200510011)
河南省科学院基金项目(220605114)。
