摘要
目的探索长春瑞滨(vinorelbine,NVB)联合阿霉素(adriamycin,PLD)后对人乳腺癌MCF-7细胞的体外抗癌效果及相关机制研究。方法CCK-8实验检测NVB和PLD单独及联用对乳腺癌细胞增殖的影响,流式细胞术检测细胞凋亡及活性氧(reactive oxygen species,ROS)水平的变化;Western blot实验检测蛋白表达。结果CCK-8结果显示与空白对照组相比,长春瑞滨处理组、阿霉素处理组及联合处理组的抑制率分别为27.6%、31.2%及65.4%,联合处理组与NVB组和PLD组相比差异有统计学意义(P=0.005 vs 0.001)。流式细胞术结果显示,各组凋亡细胞比例分别为3.54%、16.95%、15.01%及32.24%,联合处理组与NVB组和PLD组相比差异具有统计学意义(P=0.006 vs 0.005)。各组活性氧水平为1、1.03、1.06及1.57,联合处理组与NVB组和PLD组相比差异具有统计学意义(P=0.008 vs 0.007);Western blot结果表明NVB与PLD联用后p-ERK和p-STAT3的表达量降低,抑制ERK/STAT3信号通路。结论NVB与PLD联用能高效低毒的促进乳腺癌细胞的凋亡、抑制乳腺癌细胞增殖协同发挥抗癌作用,其作用机制可能是通过上调细胞中ROS水平,进而抑制ERK/STAT3通路的激活有关。
Objective To investigate the in vitro anti-cancer effect of Vinorelbine(NVB)combined with adriamycin(PLD)on human breast cancer MCF-7 cells and related mechanisms.Methods The effects of NVB and PLD alone or in combination on the proliferation of breast cancer cells were detected by CCK-8 experiment.Flow cytometry was used to detect cell apoptosis and changes in reactive oxygen species(ROS)levels.Western blot experiment was carried out to detect protein expression.Results The results of CCK-8 showed that compared with the blank control group,the inhibition rates of the vinorelbine treatment group,the adriamycin treatment group and the combined treatment group were 27.6%,31.2%and 65.4%,compared with the NVB group and PLD group,the difference between the combined treatment group was statistically significant(P=0.005 vs 0.001).The results of flow cytometry showed that the proportion of apoptotic cells in each group was 3.54%,16.95%,15.01%and 32.24%,compared with the NVB group and PLD group,the difference between the combined treatment group was statistically significant(P=0.006 vs 0.005).The levels of reactive oxygen species in each group were 1,1.03,1.06 and 1.57,compared with the NVB group and PLD group,the difference between the combined treatment group was statistically significant(P=0.008 vs 0.007).Western blot results showed that the expression of p-ERK and p-STAT3 decreased after the combination of NVB and PLD,which inhibited the ERK/STAT3 signaling pathway.Conclusions The combination of NVB and PLD can promote the apoptosis of breast cancer cells and inhibit the proliferation of breast cancer cells with high efficiency and low toxicity.Its mechanism of action may be related to the up-regulation of ROS levels in cells,thereby inhibiting the activation of the ERK/STAT3 pathway.
作者
王亚珏
赵兴娟
张静
杨璇
高润芳
Wang Yajue;Zhao Xingjuan;Zhang Jing;Yang Xuan;Gao Runfang(Department of Breast Surgery,Shanxi Provincial People’s Hospital,Taiyuan 030012,China)
出处
《中华内分泌外科杂志》
CAS
2022年第2期160-163,共4页
Chinese Journal of Endocrine Surgery
基金
山西省卫生计生委科研课题(201601019)。
