摘要
以分布在不同海拔(2279、3273、4452 m)的喜马拉雅旱獭(Marmota himalayana)为供试材料,通过二代测序技术对线粒体基因组序列进行比较分析,挖掘喜马拉雅旱獭低氧适应相关基因。结果表明:不同海拔喜马拉雅旱獭线粒体序列长度之间并无差异,但蛋白质编码基因突变位点个数随海拔增加而增多,ND5基因的突变位点个数和非同义突变位点个数都为最多,其Ka/Ks>1,表明ND5基因存在正选择效应,可作为适应高海拔低氧环境的候选基因。与玉树(海拔4452 m)相比,乐都(海拔2279 m)和黄南(海拔3273 m)的喜马拉雅旱獭亲缘关系相对较近。喜马拉雅旱獭13个蛋白质编码基因的核苷酸序列共编码氨基酸3812个,去掉起始密码子和终止密码子后共编码氨基酸3802个。在20种氨基酸中,Leu的使用率最高,达16.18%,Gys的使用率最低,仅有0.71%。RSCU值>1的密码子有32种,RSCU值<1的密码子有32种,其中AGG的RSCU为0。
The mitochondrial genome sequences of Marmota himalayana at different elevations(2,279 m,3,273 m,4,452 m)were compared and analyzed by second-generation sequencing technology,and the genes related to hypoxia adaptation were explored.The results showed that there was no difference in mitochondrial sequence length among M.himalayana at different altitudes,but the number of mutation sites of protein-coding gene increased with the increasing alti-tude.The number of mutation sites and non-synonymous mutation sites of ND5 gene was the largest,and the Ka/Ks of ND5 gene was higher than 1,indicating the positive selection effect of ND5 gene.It could be used as a candidate gene for adaptation to high altitude and low oxygen environment.Compared with Yushu,the Himalayan marmots in Ledu and Huangnan were relatively closely related.The nucleotide sequences of 13 protein-coding genes encoded 3,812 amino acids,including 3,802 amino acids after removing the start and stop codons.Among the 20 amino acids,the utilization rate of Leu was the highest(16.18%),the utilization rate of Gys was the lowest(0.71%),There were 32 codons with RSCU>1 and 32 codons with RSCU<1,among which the RSCU of AGG was 0.
作者
南新营
李优
李耀东
NAN Xinying;LI You;LI Yaodong(Qinghai University,Xining,810016,China)
出处
《野生动物学报》
北大核心
2022年第2期345-353,共9页
CHINESE JOURNAL OF WILDLIFE
基金
国家自然科学基金项目(31260259)
青海省自然科学基金项目(2018-ZJ-775)。
