摘要
目的了解绍兴市手足口病病毒型别分布,为手足口病防治工作提供参考。方法收集2018—2020年绍兴市手足口病监测哨点医院14岁以下手足口病病例的肛拭子/咽拭子标本,采用实时荧光定量PCR和BLAST序列比对测定肠道病毒型别,描述性分析肠道病毒型别构成及流行时间分布。结果2018—2020年收集肛拭子/咽拭子标本1267份,检出阳性661份,阳性率为52.17%。其中肠道病毒71型(EV71)70份,阳性率为5.52%;柯萨奇病毒A组16型(CoxA16)208份,阳性率为16.42%;CoxA6型239份,阳性率为18.86%;CoxA10型23份,阳性率为1.82%;其他型别121份,成功测序84份,检出CoxA2、CoxA4和CoxA5等10种型别。2018—2020年EV71型阳性率分别为14.08%、0.72%和1.84%,2018年4—7月为检出高峰;CoxA16型阳性率分别为10.98%、26.57%和11.98%,2019年4—8月和11—12月为检出高峰;CoxA6型阳性率分别为10.50%、14.73%和30.88%,2020年5—12月为检出高峰。结论2018—2020年绍兴市手足口病的主要肠道病毒型别为EV71、CoxA16和CoxA6型,且CoxA6型检出有上升趋势。
Objective To investigate the genotypes of enteroviruses causing hand,foot and mouth disease(HFMD)in Shaoxing City,so as to provide insights into HFMD control.Methods The anal and pharyngeal swab specimens were collected from HFMD cases under 14 years of age reported by sentinel hospitals of HFMD in Shaoxing City from 2018 to 2020.The enterovirus genotypes were determined by quantitative real-time PCR(qPCR)assay and sequenced by BLAST,and the constitution and temporal distribution of enterovirus genotypes were descriptively analyzed.Results A total of 1267 anal and pharyngeal swab specimens were collected from children with HFMD,and 661 specimens were positive for enteroviruses,with a positive rate of 52.17%.Among the 661 enterovirus-positive specimens,70 specimens were tested positive for EV71(5.52%),208 specimens tested positive for CoxA16(16.42%),239 specimens tested posi⁃tive for CoxA6(18.86%);23 specimens tested positive for CoxA10(1.82%),and 121 specimens tested positive for oth⁃er 10 genotypes,including CoxA2,CoxA4,and CoxA5.The prevalence rates of EV71 were 14.08%,0.72%and 1.84%from 2018 to 2020,and the detection of EV71 peaked during the period between April and July,2018.The prevalence rates of CoxA16 were 10.98%,26.57%and 11.98 from 2018 to 2020,and the detection of CoxA16 peaked during the period between April and August,and between November and December,2019.The prevalence rates of CoxA6 were 10.50%,14.73%and 30.88%from 2018 to 2020,and the detection of CoxA6 peaked during the period between May and December,2020.Conclusions EV71,CoxA16 and CoxA6 were predominant enterovirus genotypes causing HFMD in Shaoxing City from 2018 to 2020,and the detection of CoxA6 appeared a tendency towards a rise.
作者
蒋卓婧
葛小萍
陈金堃
王吉玲
JIANG Zhuojing;GE Xiaoping;CHEN Jinkun;WANG Jiling(Microbiological Laboratory,Shaoxing Center for Disease Control and Prevention,Shaoxing,Zhejiang 312071,China;Xiaoshan Center for Disease Control and Prevention,Hangzhou,Zhejiang 311200,China)
出处
《预防医学》
2022年第5期507-510,共4页
CHINA PREVENTIVE MEDICINE JOURNAL
关键词
手足口病
肠道病毒
基因型别
hand,foot and mouth disease
enteroviruses
genotype
