摘要
为研究长白山紫苏籽油优化提取条件及其抗炎活性,采用响应面优化水酶法提取长白山紫苏籽油的工艺条件,并初步研究其对小鼠的抗炎活性。结果表明:长白山紫苏籽油的最佳提取条件为料液比1∶10(g/mL)、碱性蛋白酶加酶量3%、酶解温度45℃、酶解时间3 h。此条件下,提取率为76.49%。通过其对小鼠毛细血管通透性及耳肿胀实验发现,长白山紫苏籽油低、中和高剂量组能显著抑制醋酸致小鼠毛细血管通透性增加,且紫苏籽油各剂量组对二甲苯致小鼠耳廓肿胀有显著或极显著的抑制作用,表明长白山紫苏籽油具有良好的抗炎活性。
To study the optimal extraction conditions and anti-inflammatory activity of Perilla seed oil from Changbai Mountain.Response surface methodology(RSM)was used to optimize the aqueous enzymatic extraction of Perilla seed oil from Changbai Mountain,and its anti-inflammatory activity against mice was explored.The results showed that the optimal extraction conditions were as follows:solid-to-liquid ratio of 1∶10(g/mL),alkaline protease content of 3%,and enzymatic hydrolysis temperature and time of 45℃and 3 h.Under the above conditions,the extraction yield of Perilla seed oil from Changbai Mountain was 76.49%.The capillary permeability test and ear swelling test in mice found that the low,medium and high dose groups of Changbai Mountain Perilla seed oil could significantly inhibit the increase of capillary permeability induced by acetic acid in mic and in all dose groups of Changbai Mountain Perilla seed oil,xylene-induced mice auricle swelling was marked or extremely marked inhibited,indicating that Perilla seed oil from Changbai Mountain had good anti-inflammatory activity.
作者
吕萍
LV Ping(College of Food Engineering,Jilin Agricultural Science and Technology University,Jilin 132101,Jilin,China)
出处
《食品研究与开发》
CAS
北大核心
2022年第9期96-102,共7页
Food Research and Development
基金
吉林省教育厅科学技术研究项目(JJKH20210405KJ)。
关键词
响应面
紫苏籽油
水酶法
提取工艺
抗炎活性
response surface
Perilla seed oil
aqueous enzyme
extraction process
anti-inflammatory activity
