摘要
In the present study,three previously undescribed steroidal glycoalkaloids(compounds 1–3)were isolated from Solanum lyratum.Their structures were elucidated based on comprehensive spectroscopic data.Their anti-angiogenesis and anti-metastatic activities were evaluated by MTT and wound-healing assays,respectively.Tumor-derived vascular endothelial cells(TdECs),obtained by co-culture of A549 and human umbilical vein endothelial cells(HUVECs),were treated with compounds 1–3.Results showed that compounds 1–3 significantly inhibited the migration of TdECs at 25μM despite the weak cytotoxic activities,which indicated that the compounds exerted anti-tumor activities by inhibiting metastasis,rather than directly inhibiting the proliferation of TdECs.
本研究从白英中分离得到3个未被报道的甾体糖苷生物碱(化合物1–3),借助综合光谱分析,阐明了它们的结构。通过MTT和划痕愈合实验分别评价其抗肿瘤血管生成和转移活性,为更好的模拟体内肿瘤环境,将人非小细胞肺癌细胞株A549与人脐静脉内皮细胞共培养获得肿瘤血管内皮细胞(TdECs)模型,用化合物1–3分别处理TdECs。结果显示,尽管它们对TdECs的细胞毒活性相对较弱,但在25μM时均能明显抑制TdECs的迁移。这些结果表明,化合物1–3并不是通过影响肿瘤血管内皮细胞的增殖直接作用于血管生成,而是通过抑制肿瘤细胞的转移发挥抗肿瘤活性。
基金
National Natural Science Foundation of China(Grant No.81872989).
