摘要
目的调查温岭市女性宫颈炎患者人乳头瘤病毒(HPV)的感染情况及其基因型的分子流行病学特点。方法选取宫颈炎患者的宫颈脱落细胞2293份,采用多重PCR毛细管电泳技术对HPV E6/E7 DNA进行分型,包括18种高危型别和7种低危型别,分析25种基因型的流行病学特点。结果2293份样本中共检出HPV感染793份,总感染率为34.58%,感染率以<20岁组最高;单一型别感染率为66.46%,多重型别感染率为33.54%;高危型HPV占总基因型的81.77%,前5位高危型HPV是HPV52、HPV58、HPV16、HPV53、HPV51;低危型HPV占18.23%,其中以HPV81感染率最高,其次是HPV42;多重型别感染中以二重感染为主,共检出167例,三重感染共检出64例,四重感染共检出23例,五重感染共检出10例,七重感染共检出2例。结论温岭市女性宫颈炎患者以高危型HPV52型感染为主,其次是HPV58、HPV16、HPV53、HPV51,多重感染的患者以二重感染为主,为疾病的防控、HPV疫苗的研发及宫颈癌的防治提供了理论依据。
Objective To investigate the infection of 25 human papillomavirus genotypes and the molecular epidemiological characteristics in women with cervicitis.Methods A total of 2293 cases of cervical exfoliated cells from patients with cervicitis were selected,and HPV E6/E7 DNA was typed by multiplex PCR capillary electrophoresis,including 18 high-risk and 7 low-risk types,and then the molecular epidemiologic characteristics were studied.Results The total infection rate of HPV was 34.58%.Among different stages of the year,theinfection rate was the highest in younger than 20 years old group.The infection rate of single type and multiple types were 66.46%and 33.54%.The high-risk HPV accounted for 81.77%of the total genotypes,the most commonly detected genotypes were HPV 52,HPV 58,HPV 16,HPV 53,HPV 51,the low-risk HPV accounted for 18.23%,the most common genotypes were HPV 81 and HPV 42.There were 167 cases had superinfections,64 cases had triple infections,23 cases had quadruple infections,10 cases had five-storeyed infections and 2 cases had seven storeyed infections.Conclusion The high-risk HPV 52 is the main HPV genotype of cervicitis,followed by HPV 58,HPV 16,HPV 53,HPV 51.Superinfection is the main type of multiple infection.It provides a theoretical basis for the development of the HPV vaccine and the prevention and treatment of cervix cancer.
作者
张婷
李蒙佳
陈瑛
郭洁洁
ZHANG Ting;LI Mengjia;CHEN Ying(Department of Clinical Laboratory,The First People's Hospital of Wenling,The Affliated Wenling Hospital of Wenzhou Medical University,Wenling 317500,China)
出处
《全科医学临床与教育》
2022年第4期302-305,共4页
Clinical Education of General Practice
基金
浙江省自然科技项目(LQ19H090001)
台州市社会发展科技计划项目(20ywb135)
温岭市社会发展科技项目(2021S00148)。
关键词
人乳头瘤病毒
宫颈炎
基因型
感染率
human papilloma virus
cervicitis
genotype
infection rate
