摘要
本研究从康普茶中分离得到一种新型细菌素植物乳杆菌素#SLG10,对革兰氏阳性菌和革兰氏阴性菌,包括多重耐药菌株均有抗菌活性。本文以一种新的方法,即生物色谱与反相高效液相色谱(RP-HPLC)相结合,用于从乳酸菌的胞外无细胞发酵液中筛选和纯化获得植物乳杆菌素#SLG10。由N-测序确定氨基酸序列为Asn-Ile-Val-Trp-Gln-Leu-Ile-Gly-Leu-Pro-Ala-Gln-A1。
In this study,a new bacteriocin Phytolactoxin#SLG10 was isolated from Kombucha,which has antibacterial activity against Gram-positive bacteria and Gram-negative bacteria,including multi-drug resistant strains.In this paper,a novel method,namely biochromatography combined with reversed-phase high performance liquid chromatography(RP-HPLC),was used to screen and purify the plant lactobacillus#SLG10 from the extracellular cell-free fermentation broth of lactic acid bacteria.The amino acid sequence was Asn-Ile-Val-Trp-Gln-Leu-Ile-Gly-Leu-Pro-Ala-Gln-Al,as determined by N-sequencing.
作者
刘哲
王金泽
黄一刚
任婷
金文刚
裴金金
Liu Zhe;Wang Jinze;Huang Yigang;Ren Ting;Jin Wengang;Pei Jinjin(School of Biological Science and Engineering,Shaanxi University of Technology,Shaanxi,723000)
出处
《当代化工研究》
2022年第8期162-164,共3页
Modern Chemical Research
基金
2021年陕西理工大学全国大学生生命科学竞赛资助项目“硒的营养功能特性及研究开发现状”(项目编号:65906)
2020年陕西省科技厅基金项目“源自全球主要天然富硒区的富硒乳酸菌筛选及其体内生物活性和营养功能研究”(项目编号:SX2020-WGZJ-005)。
关键词
植物乳杆菌
细菌素
纯化
鉴定
lactobacillus plantarum
bacteriocin
purification
identify
