摘要
目的:研究壳寡糖对酒精诱导的新生大鼠脑组织氧化应激损伤和细胞凋亡的抑制作用。方法:将56只SD新生大鼠随机分为正常组(NC)、药理组(COS)、模型组(Mod)、壳寡糖干预组(COS+Mod),通过对SD新生大鼠进行灌胃酒精干预造模。通过体质量变化、病理学观察、氧化应激损伤和凋亡相关蛋白表达情况评估壳寡糖的保护作用。结果显示,壳寡糖干预后能缓解酒精诱导新生大鼠的体质量增长缓慢,缓解脑神经细胞坏死变形,提高脑组织中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)水平和总抗氧化能力(total antioxidant capacity,T-AOC),明显降低脑组织的丙二醛(malondiadehyde,MDA)含量。壳寡糖干预能明显改善酒精造成的新生大鼠脑部氧化应激损伤,降低促凋亡蛋白Cleaved Caspase-3的表达。结论:壳寡糖对酒精诱导的新生大鼠脑损伤具有明显的保护作用,其机制可能与壳寡糖能够缓解酒精诱导的脑组织氧化应激损伤和抑制神经细胞凋亡有关。
The purpose of this study was to investigate the protective effect of chitooligosaccharide(COS)against alcohol-induced oxidative stress injury and neuronal cell apoptosis in the brain of neonatal rats.In total,56 neonatal SD rats were randomly divided into four groups:normal control(NC),COS,model(MOD),and COS+MOD.The effect of COS on body mass change,histopathology,oxidative stress injury and the expression of apoptosis-associated proteins was evaluated.The results showed that oral administration of alcohol slowed down body mass gain and caused necrosis and deformation of brain nerve cells,but this effect was relieved by intervention with COS.Additionally,intervention with COS increased the levels of superoxide dismutase(SOD)activity,glutathione(GSH)content and total antioxidant capacity(T-AOC),and significantly reduced malondiadehyde(MDA)level in brain tissue.COS significantly relieved oxidative stress damage caused by ethanol exposure in the brain of neonatal rats,and reduced the expression of cleaved caspase-3.These results indicated that COS protects against brain damage induced by ethanol in neonatal rats,and the underlying mechanism may be related to the fact that COS can alieve ethanol-induced oxidative stress injury of brain tissue and inhibit neuronal cell apoptosis.
作者
王斌
陈逸伦
夏文水
WANG Bin;CHEN Yilun;XIA Wenshui(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Wuxi 214122,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第7期105-111,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(31800679)
“十三五”国家重点研发计划重点专项(2018YFD0901104)。
关键词
壳寡糖
酒精诱导脑损伤
氧化应激
凋亡
chitooligosaccharide
ethanol-induced brain damage
oxidative stress
apoptosis
