摘要
【目的】探究外源褪黑素在生菜应对高温胁迫中的作用,使褪黑能更好的应用到生菜生产。【方法】以热敏感生菜品种‘北散生3号’为试验材料,采用水培方式,叶面喷施50μmol/L的褪黑素(MT),研究外源MT对高温胁迫下生菜生长、叶绿素含量、膜透性及抗氧化酶活性的影响。【结果】高温胁迫下,生菜幼苗地上部鲜质量、地下部鲜质量、相对含水量减少。生菜幼苗叶绿素a、b含量及总量显著增加;丙二醛(MDA)含量增加;过氧化氢酶(CAT)、过氧化物酶(POD)活性先升后降;超氧化物歧化酶(SOD)显著下降。外源喷施MT生菜地上部鲜质量、地下部鲜质量、地上部干质量、相对含水量增加;MDA含量减少;SOD、CAT酶活性显著增加。【结论】叶面喷施外源褪黑素能够有效降低生菜膜透性,提高抗氧化系统活性,缓解高温胁迫对生菜生长的不利影响。
[Objective]High temperature is an important abiotic stress in lettuce production. The mechanism of exogenous melatonin(MT)-regulated lettuce antioxidant enzyme system with high temperature stress is not clearly understood.[Methods]The experimental material was the heat-sensitive variety ’No. 3 Beisansheng’ lettuce by hydroponics, and 50 μmol/L MT was sprayed on leaves. Under high temperature stress, the effects of exogenous MT on the growth of lettuce chlorophyll content, membrane permeability and antioxidant enzyme activity were studied.[Result]Under high temperature stress, shoot fresh biomass of lettuce, root fresh biomass, the plant water content were reduced. The contents and total amount of chlorophyll a and b in lettuce seedlings were significantly increased. The malondialdehyde(MDA) content was increased. Catalase(CAT) and peroxidase(POD) activities were increased first and then decreased;Superoxide dismutase(SOD) activity was decreased significantly. The shoot fresh biomass of lettuce, root fresh biomass, shoot dry biomass, the plant water content of lettuce were increased after MT treatment. The content of MDA decreased. The activities of SOD and CAT were significantly increased by MT treatment.[Conclusion]The spraying of exogenous melatonin on the leaf surface can effectively reduce membrane permeability, improve the activity of antioxidant system and alleviate the adverse effects of high temperature on lettuce growth.
作者
王译
韩莹琰
郝敬虹
刘超杰
范双喜
WANG Yi;HAN Yingyan;HAO Jinghong;LIU Chaojie;FAN Shuangxi(College of Plant Science&Technology,Beijing University of Agriculture,Beijing 102206,China)
出处
《北京农学院学报》
2022年第2期45-49,共5页
Journal of Beijing University of Agriculture
基金
叶类蔬菜产业技术体系北京市创新团队(BAIC07)。
关键词
生菜
高温胁迫
褪黑素
抗氧化
lettuce
high temperature stress
melatonin
antioxidant
