摘要
以蓝靛果为原料,采用AB-8大孔树脂联合高速逆流色谱法(AB-8 Macroporous resin combined with high speed counter-current chromatography,AB-8MR-HSCCC)分离纯化蓝靛果花色苷,并结合紫外、高效液相色谱、液质联用和核磁鉴定花色苷结构。结果表明:确定正丁醇-甲基叔丁基醚-乙腈-水-三氟乙酸(2:2:1:5:0.02,v/v)为分离蓝靛果花色苷的HSCCC溶剂体系。蓝靛果粗提取物经AB-8MR-HSCCC分离后得到4个分离组分(F1、F2、F3和F4),其中F1和F4为非花色苷类物质;F2和F3在波长280 nm和520 nm左右分别有紫外和可见光的吸收特性,并最终鉴定F2和F3分别为矢车菊-3-O-葡萄糖苷和矢车菊-3-O-芸香糖苷,其纯度分别为93.81%和90.14%。研究结果为花色苷单体的分离纯化提供了一种有效可行的方法。
Anthocyanins from Lonicera edulis were separated and purified by AB-8 macroporous resin combined with high speed counter-current chromatography(AB-8MR-HSCCC),and identified by UV,HPLC,HPLC-MS,and NMR.The results showed that the HSCCC solvent system for the separation of anthocyanins from Lonicera edulis was 1-butanol-methyl tert-butyl ether-acetonitrile-water-trifluoroacetic acid(2:2:1:5:0.02,v/v).Four separated components(F1,F2,F3,and F4) were obtained from the crude extract of Lonicera edulis by AB-8MR-HSCCC,and F1 and F4 were non anthocyanins.F2 and F3 have UV and visible absorption characteristics at wavelengths of about 280 nm and 520 nm,respectively.Finally,F2 and F3 are identified as cyanidin-3-O-glucoside and cyanidin-3-O-rutoside respectively,and their purity was 93.81% and 90.14%,respectively.The results provide an effective and feasible method for the separation and purification of anthocyanin monomers.
作者
韩蕈泽
HAN Xunze(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)
出处
《食品科技》
CAS
北大核心
2022年第3期231-237,共7页
Food Science and Technology
基金
北京市大学生创新创业项目(202103010629)。
关键词
AB-8大孔树脂
高速逆流色谱
蓝靛果
分离纯化
花色苷
AB-8 macroporous resin
high-speed counter-current chromatography
Lonicera edulis
separation and purification
anthocyanins
