摘要
目的探讨如何利用腹腔引流管载体建立小鼠腹腔铜绿假单胞菌(PA)生物被膜(BF)感染的模型,为进一步研究PA BF体内感染的致病性、耐药机制及其防治提供研究平台。方法利用临床常用的腹腔引流管切成圆片在体外预先建立PA BF感染的载体,然后把载体植入小鼠的腹腔造成BF感染的动物模型。1 d后评估小鼠腹腔的感染情况、细菌学和病理学的改变等。结果小鼠腹腔载体植入1 d后取出引流管载体,可见PAO1组载体表面有生物被膜样结构存在,并培养出铜绿假单胞菌活菌,腹腔载体周围包裹组织呈现出急性炎症改变;术后第2天,PAO1组小鼠比空白对照组小鼠体表温度较前1 d增高更明显,体重较前1 d降低更明显,差异有统计学意义(P<0.05)。结论体外制作的腹腔引流管PA生物膜载体植入体内可诱发小鼠腹腔PA BF感染,模拟体内留置导管引起的PA BF感染的情况,对这种难治性感染的基础研究提供了可靠的动物模型。
Objective To investigate how to establish a model of Pseudomonas aeruginosa(PA)biofilm(BF)infection in the mouse abdominal cavity using an abdominal drainage tube carrier,so as to provide a platform for further study of the pathogenicity,drug resistance mechanism and prevention and treatment of PA-BF infection in vivo.Methods The carrier for PA-BF infection was established in vitro by using sliced discs of the commonly used abdominal drainage tube and then the carrier was implanted into the abdominal cavity of mice to create an animal model of BF infection.1 day later,the infection status,bacteriological and pathological changes in the abdominal cavity of mice were evaluated.Results When the d rainage tu be carrier was removed 1 day after implantation,BF-like structures were observed on the surface of the carrier in the PAO1 group,live PA were cultured,and acute inflammatory changes were observed in the surrounding tissues of the carrier in the abdominal cavity.On the second day after surgery,the mice in the PAO1 group showed a more significant increase in body surface temperature and a more significant decrease in body weight than those in the blank control group on the previous day,with statistically significant differences(P<0.05).Conclusion The in vivo implantation of PA-BF carrier made by using an abdominal drainage tube in vitro can induce abdominal PA-BF infection in mice and simulate the situation of PA-BF infection caused by indwelling catheters in vivo,thus providing a reliable animal model for the basic study of this r efractory infection.
作者
刘晓岚
周志坚
张真强
秦海洸
刘晓庆
荆雪宁
孔晋亮
宋志军
吴红
LIU Xiaolan;ZHOU Zhijian;ZHANG Zhenqiang;QIN Haiguang;LIU Xiaoqing;JING Xuening;KONG Jinliang;SONG Zhijun;WU Hong(Department of Medicine,Shandong College of Traditional Chinese Medicine,Shandong,Yantai 264199,China;Department of General Surgery,Yidu Central Hospital of Weifang,Shandong,Qingzhou 262500,China;Department of Respiratory Medicine and Intensive Care Unit,Liuzhou People’s Hospital,Guangxi,Liuzhou 545006,China;Department of Respiratory Medicine and Intensive Care Unit,the First Affiliated Hospital of Guangxi Medical University,Guangxi,Nanning 530021,China;Department of Clinical Microbiology,Hospital South West Jutland,University Hospital of Southern Denmark,Denmark,Esbjerg;Department of Clinical Microbiology,Rigshospitalet,University Hospital of Copenhagen,Denmark,Copenhagen)
出处
《中国医药科学》
2022年第7期31-35,共5页
China Medicine And Pharmacy
基金
山东省医药卫生科技发展计划项目(2017WS070)
山东省中医药科技项目(2021M211)
山东中医药高等专科学校教研科研课题(2018ky24)。
