摘要
铁离子是生物体内重要的元素之一。设计合成了基于罗丹明B-甘氨酸甲酯的Turn-On型Fe^(3+)荧光探针,利用^(1)HNMR和LC-MS对其结构进行表征。研究结果表明:探针中加入Fe^(3+)后展现出明显的荧光信号,当加入其他分析物(Fe^(2+)、Hg^(2+)、Zn^(2+)、Ba^(2+)、Al^(3+)、Co^(2+)、Cd^(2+)、K^(+)、Na^(+)、Mn^(2+)、Pd^(2+)、Pb^(2+)、Ca^(2+)、Ni^(2+)、Cu^(2+)、Ag^(+))后,探针荧光信号几乎无明显变化,探针对Fe^(3+)具有卓越地选择性,不受其他竞争分析物的干扰;根据反应动力学分析确定探针识别Fe^(3+)是非常迅速的过程,准一级反应动力学常数k为4.97×10^(-2) s^(-1);依据荧光光谱滴定实验可知探针溶液的荧光强度变化量与Fe^(3+)浓度的改变量呈现良好的线性关系,并且计算出最低检测浓度为0.21μmol·L^(-1)。最后,成功地将探针应用于A549细胞体内进行Fe^(3+)浓度的测定。
Iron ion is one of the most important elements in living organisms.In this paper,a Turn-On Fe^(3+)fluorescent probe based On Rhodamine B-Glycine methyl ester was designed and synthesized,and its structure was characterized by ^(1)H-NMR and LC-MS.The results show that the Fe^(3+)was added into the probe,the fluorescence signal was obvious.When other analytes(Fe^(2+)、Hg^(2+)、Zn^(2+)、Ba^(2+)、Al^(3+)、Co^(2+)、Cd^(2+)、K^(+)、Na^(+)、Mn^(2+)、Pd^(2+)、Pb^(2+)、Ca^(2+)、Ni^(2+)、Cu^(2+)、Ag^(+))were added,the fluorescence signal of the probe was almost unchanged,and the probe had excellent selectivity for Fe^(3+),which was not the interference of other competing analytes;According to the kinetic analysis of the reaction,it was determined that the probe recognition of Fe^(3+) was a very rapid process,and the kinetic constant(k)of the quasi-first order reaction was 4.97×10^(-2) s^(-1).The fluorescence spectrum titration experiment showed that the fluorescence intensity of the probe solution with the Fe^(3+) concentration was a good linear relationship,and the low of detection was calculated to be 0.21μmol·L^(-1).Finally,the probe was successfully applied to the determination of Fe^(3+) concentration in A549 cells.
作者
李宏达
周莹健
吴晓璟
王岩
LI Hong-da;ZHOU Ying-jian;WU Xiao-jing;WANG Yan(Department of Forensic Chemistry,Criminal Investigation Police University of China,Shenyang 110035,China;Institute of Functional Molecules,Shenyang University of Chemical Technology,Shenyang 110142,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2022年第4期795-801,共7页
Chemical Research and Application
基金
国家自然科学基金青年项目(21804140)资助
辽宁省自然科学基金项目(2020-MS-131)资助
公安部技术研究计划项目(2020JSYJC26)资助。
