摘要
Polyetheretherketone(PEEK)has been widely used as orthopedic and dental materials due to excellent mechanical and physicochemical tolerance.However,its biological inertness,poor osteoinduction,and weak antibacterial activity make the clinical applications in a dilemma.Inspired by the mussel adhesion mechanism,here we reported a biomimetic surface strategy for rational integration and optimization of anti-infectivity and osteo-inductivity onto PEEK surfaces using a mussel foot proteins(Mfps)-mimic peptide with clickable azido terminal.The peptide enables mussel-like adhesion on PEEK biomaterial surfaces,leaving azido groups for the further steps of biofunctionalizations.In this study,antimicrobial peptide(AMP)and osteogenic growth peptide(OGP)were bioorthogonally clicked on the azido-modified PEEK biomaterials to obtain a dual-effect of host defense and tissue repair.Since bioorthogonal clicking allows precise collocation between AMP and OGP through changing their feeding molar ratios,an optimal PEEK surface was finally obtained in this research,which could long-term inhibit bacterial growth,stabilize bone homeostasis and facilitate interfacial bone regeneration.In a word,this upgraded mussel surface strategy proposed in this study is promising for the surface bioengineering of inert medical implants,in particular,achieving rational integration of multiple biofunctions to match clinical requirements.
基金
supported by the National Key Research and Development Program of China(2019YFA0112000)
Research and Development of Biomedical Materials and Substitution of Tissue and Organ Repair under the National Key R&D Program(2016YFC1101505)
the National Natural Science Foundation of China(82072425,82072498,81873991,81073990,21875092,31922040 and 81672238)
the Young Medical Talents of Jiangsu Province(QNRC2016751)
the Natural Science Foundation of Jiangsu Province(BK20180001)
the Innovation and Entrepreneurship Program of Jiangsu Province
the“Six Talent Peaks”program of Jiangsu Province(2018-XCL-013)
the Basic Applied Research Program of Suzhou City(SYS2018032,KJXW2017009)
the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
the Special Project of Diagnosis and Treatment for Clinical Diseases of Suzhou(LCZX202003).
