摘要
目的比较3D打印和传统铸造钴铬合金的理化性能及生物学影响。方法实验组和对照组分别用3D打印技术中的选择性激光熔融(SLM)和传统铸造技术,制作钴铬合金试件各10个,采用洛氏硬度计HR-150A和金相显微镜对两组分别进行洛氏硬度测定和金相观察,并在自腐蚀电位下对金属试件进行极化曲线测定。在无菌环境下制备试件的浸提液,并将其分为阴性对照组、3D打印钴铬合金浸提液、铸造钴铬合金浸提液(每组5个),利用CCK-8试剂测定浸提液对小鼠成纤维细胞L-929的细胞毒性,运用AnnexinⅤ-FITC/PI荧光染色检测细胞凋亡,用流式细胞仪计算不同组别的凋亡率。结果3D打印组、铸造组的洛氏硬度分别为70.6±0.1、68.5±0.2,3D打印组强于铸造组,有统计学意义(P<0.005)。3D打印钴铬合金组织没有第二相;铸造组织为树枝晶,第二相强化。3D打印和铸造组钴铬合金在自腐蚀电位下的电流密度分别为4.3381×10^(-9) A/cm^(2)和3.3785×10^(-8)A/cm^(2),铸造组电流密度高于3D打印组。在24、48、72 h,阴性对照组的OD平均值约为0.44、1.14、1.50,3D组的OD平均值约为0.39、0.95、1.09,铸造组的OD平均值约为0.35、0.76、1.01,各组间的细胞毒性差异均有统计学意义(P<0.05),铸造组细胞毒性最强,各组均随着时间延长细胞毒性逐渐增强。经流式细胞仪分析三组均以正常细胞为主,凋亡率分别为(2.497±0.121)%、(3.513±0.055)%、(5.407±0.401)%,各组间均有统计学意义(P<0.01)。结论与传统铸造合金相比,3D打印技术制造的钴铬合金具有更好的机械性能和生物相容性,在临床有更好的应用前景。
Objective To compare physicochemical properties and biocompatibility of cobalt-chromium(Co-Cr)alloy in 3D printing technology and conventional alloy casting technique.Methods The experimental group and the control group each contained 10 Co-Cr alloy specimens fabricated by selective laser melting(SLM)and traditional casting technology respectively.The Rockwell hardness test-er HR-150A and the metallurgical microscope were used to compare two groups respectively,and then polarization curve was measured on metal specimens under self-corrosion potential.Leaching solution of the test piece was prepared in a sterile environment,and was di-vided into negative control group,3D printing Co-Cr alloy group and cast Co-Cr alloy group(5 per group).Using CCK-8 reagent,the cytotoxicity of the extract to mouse fibroblast L-929 was determined,and the apoptosis was detected by AnnexinⅤ-FITC/PI fluores-cence staining;the apoptosis rate of different groups was calculated by flow cytometry.Results The Rockwell hardness of the 3D printing group and the casting group were 70.6±0.1 and 68.5±0.2,respectively.The 3D printing group was stronger than the casting group,and the difference between two groups was statistically significant(P<0.005).The 3D printed Co-Cr alloy structure didn’t have a second phase;the cast structure was dendritic,and the second phase was strengthened.The current densities of the 3D printing and casting groups of Co-Cr alloys under self-corrosion potential were 4.3381×10^(-9)A/cm^(2)and 3.3785×10^(-8) A/cm^(2),respectively.The cur-rent density of the casting group was higher than that of the 3D printing group.At 24,48 and 72 h,the average OD of the negative con-trol group was approximately 0.44,1.14,1.50,the 3D group was 0.39,0.95,1.09,the casting group was 0.35,0.76,1.01.The differences in cytotoxicity between groups were statistically significant(P<0.05).The casting group had the strongest cytotoxicity,and the cytotoxicity of each group gradually increased with time.Flow cytometry suggested primarily norm
作者
刘爽
马国武
LIU Shuang;MA Guowu(Department of Comprehensive and Emergency Treatment Clinic,Dalian Stomatological Hospital,Dalian 116021,China)
出处
《口腔医学》
CAS
2022年第3期210-214,共5页
Stomatology
关键词
3D打印
传统铸造
钴铬合金
细胞凋亡
3D printing
conventional casting
cobalt-chromium alloy
cell apoptosis
