摘要
为研究烟叶烘烤过程中烤房内挥发性成分的变化规律,采用固相微萃取-气相色谱/质谱(SPME-GC/MS)联用的方法检测烤房内和烟叶中的挥发性成分,并对检测到的挥发性成分进行分析。结果表明,在烤房内和烟叶中检测到的挥发性成分分别有76种和96种,共同检测到的挥发性成分有19种;其中烟碱和叶绿醇是烤房内和烟叶中挥发性成分含量最多的物质,总量占比分别为91.59%和92.86%,烤房内烟碱和叶绿醇相对香气活性值(ROAV)分别为1030.30和138.87;烤房内的烟碱含量和烟叶中的烟碱含量变化趋势一致,总体呈现先升高后降低并逐渐稳定的趋势,在42℃末达到最大值,并在47℃末—68℃末趋于稳定;烟叶中的叶绿醇含量在烘烤过程中呈先升高后降低的趋势,在42℃末达到最大值,烤房中的叶绿醇含量在烘烤过程中呈逐渐升高的趋势,在54℃末—68℃末趋于稳定。聚类分析结果表明,烤房内挥发性成分在开烤—42℃末变化明显,在47℃末—68℃末趋于稳定。
In order to study the change of volatile components in curing room during tobacco curing process,the volatile components in curing room and tobacco leaves were detected by solid phase microextraction combined with gas chromatography-mass spectrometry(SPME-GC/MS),and the detected volatile components were analyzed.The results showed that 76 and 96 volatile components were detected in the curing room and tobacco leaves,respectively,and 19 volatile components were detected together.Nicotine and chlorophyl were the most volatile components in curing room and tobacco leaves,and the total proportions were 91.59%and 92.86%,respectively.The relative aroma activity values(ROAV)of nicotine and chlorophyl were 1030.30 and 138.87,respectively in curing room.The nicotine content in the curing room had the same trend as that in the tobacco leaves,increasing first,then decreasing and gradually stabilizing,reaching the maximum value at the end of 42℃and stabilizing at the end of 47—68℃.The content of chlorophyl in tobacco leaves increased firstly and then decreased during the curing process,and reached the maximum value at the end of 42℃.The content of chlorophyl in curing room increased gradually during the curing process,and stabilized at the end of 54—68℃.The results of cluster analysis showed that the volatile components changed significantly from the beginning of baking to the end of 42℃and tended to be stable at the end of 47—68℃.
作者
黄涛
雷强
俞世康
何佶弦
张启莉
谢良文
母明新
景延秋
HUANG Tao;LEI Qiang;YU Shikang;HE Jixian;ZHANG Qili;XIE Liangwen;MU Mingxin;JING Yanqiu(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China;Sichuan Company of China Tobacco Corporation,Chengdu 610041,China;Sichuan Institute of Tobacco Sciences,Chengdu 615000,China;Guangyuan City Company of Sichuan Tobacco Company,Guangyuan 628000,China)
出处
《河南农业科学》
北大核心
2022年第2期169-180,共12页
Journal of Henan Agricultural Sciences
基金
中国烟草总公司四川省公司资助项目(2019410102000453)。
关键词
烟叶
烤房
挥发性成分
固相微萃取
气相色谱-质谱
Tobacco leaves
Curing room
Volatile components
Solid phase microextraction
GC-MS
